Enzymatic synthesis of deoxyribonucleic acid. xiv. further purification and properties of deoxyribonucleic acid polymerase of escherichia coli.
TLDR
Exonuclease function of DNA polymerase from Escherichia colis, discussing hydrolysis of polydeoxyribonucleotides and resistancy of oligonucleotide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1969-06-10 and is currently open access. It has received 705 citations till now. The article focuses on the topics: DNA polymerase & Oligonucleotide.read more
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AIM2 recognizes cytosolic dsDNA and forms a caspase-1 activating inflammasome with ASC
Veit Hornung,Veit Hornung,Andrea Ablasser,Andrea Ablasser,Marie Charrel-Dennis,Franz Bauernfeind,Franz Bauernfeind,Gabor Horvath,Daniel R. Caffrey,Eicke Latz,Katherine A. Fitzgerald +10 more
TL;DR: Using mouse and human cells, the PYHIN (pyrin and HIN domain-containing protein) family member absent in melanoma 2 (AIM2) is identified as a receptor for cytosolic DNA, which regulates caspase-1.
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DNA Detection Using Recombination Proteins
TL;DR: RPA couples isothermal recombinase polymerase-driven primer targeting of template material with strand-displacement DNA synthesis and achieves exponential amplification with no need for pretreatment of sample DNA, thereby establishing an instrument-free DNA testing system.
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Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
TL;DR: The colicinogenic factor E(1) (Col E( 1)) in Escherichia coli continues to replicate by a semiconservative mechanism in the presence of chloramphenicol for 10 to 15 hr, long after chromosomal deoxyribonucleic acid (DNA) synthesis has terminated.
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In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
TL;DR: A series of plasmid vectors suitable for the detection and cloning of translational control signals and 5' coding sequences of exogenously derived genes yielded hybrid proteins useful for purifying peptide sequences encoded by exogenous deoxyribonucleic acid fragments and for studies relating the structure and function of specific peptide segments.
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A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
TL;DR: A method for the purification of Escherichia coli DNA-dependent RNA polymerase which is rapid, reproducible, high in yield, and able to handle preparations using from 1 g to 3 kg of cells is described.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.