Evolutionary relationships among cyanobacteria and green chloroplasts.
TLDR
The results indicate that many diverse forms of cyanobacteria diverged within a short span of evolutionary distance and suggest that the chloroplast lineage, which includes the cyanelle of C. paradoxa, is not just a sister group to the free-living forms but rather is contained within the cyanobacterial radiation.Abstract:
The 16S rRNAs from 29 cyanobacteria and the cyanelle of the phytoflagellate Cyanophora paradoxa were partially sequenced by a dideoxynucleotide-terminated, primer extension method A least-squares distance matrix analysis was used to infer phylogenetic trees that include green chloroplasts (those of euglenoids, green algae, and higher plants) The results indicate that many diverse forms of cyanobacteria diverged within a short span of evolutionary distance Evolutionary depth within the surveyed cyanobacteria is substantially less than that separating the major eubacterial taxa, as though cyanobacterial diversification occurred significantly after the appearance of the major eubacterial groups Three of the five taxonomic sections defined by Rippka et al (R Rippka, J Deruelles, J B Waterbury, M Herdman, and R Y Stanier, J Gen Microbiol 111:1-61, 1979) (sections II [pleurocapsalean], IV [heterocystous, filamentous, nonbranching], and V [heterocystous, filamentous, branching]) are phylogenetically coherent However, the other two sections (I [unicellular] and III [nonheterocystous, filamentous]) are intermixed and hence are not natural groupings Our results not only support the conclusion of previous workers that the cyanobacteria and green chloroplasts form a coherent phylogenetic group but also suggest that the chloroplast lineage, which includes the cyanelle of C paradoxa, is not just a sister group to the free-living forms but rather is contained within the cyanobacterial radiationread more
Citations
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Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
TL;DR: Analysis of the genomic DNA from a bacterial biofilm grown under aerobic conditions suggests that sulfate-reducing bacteria, despite their anaerobicity, were present in this environment.
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Ribosomal DNA: molecular evolution and phylogenetic inference.
David M. Hillis,Michael T. Dixon +1 more
TL;DR: An analysis of aligned sequences of the four nuclear and two mitochondrial rRNA genes identified regions of these genes that are likely to be useful to address phylogenetic problems over a wide range of levels of divergence.
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Genetic diversity in Sargasso Sea bacterioplankton.
TL;DR: The phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton indicate the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community.
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Investigating Deep Phylogenetic Relationships among Cyanobacteria and Plastids by Small Subunit rRNA Sequence Analysis
TL;DR: Small subunit rRNA sequence data were generated for 27 strains of cyanobacteria and incorporated into a phylogenetic analysis of 1,377 aligned sequence positions, finding all plastids cluster as a strongly supported monophyletic group arising near the root of the cyanobacterial line of descent.
References
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Generic assignments, strain histories, and properties of pure cultures of cyanobacteria
TL;DR: Revisions are designed to permit the generic identification of cultures, often difficult through use of the field-based system of phycological classification, and are both constant and readily determinable in cultured material.
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Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses
TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
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Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
TL;DR: Comparison of the sequence of λrifd18 with sequences from other isolates of the rrB operon provides direct evidence for structural rearrangements within rRNA operons.