Journal ArticleDOI
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
TLDR
Comparison of the sequence of λrifd18 with sequences from other isolates of the rrB operon provides direct evidence for structural rearrangements within rRNA operons.About:
This article is published in Journal of Molecular Biology.The article was published on 1981-05-15. It has received 1683 citations till now. The article focuses on the topics: RRNA Operon & Ribosomal RNA.read more
Citations
More filters
Journal ArticleDOI
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
TL;DR: Phylogenetic analysis of the retrieved rRNA sequence of an uncultured microorganism reveals its closest culturable relatives and may, together with information on the physicochemical conditions of its natural habitat, facilitate more directed cultivation attempts.
Journal ArticleDOI
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
F W Studier,B A Moffatt +1 more
TL;DR: A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.
Journal ArticleDOI
Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species.
Ok-Sun Kim,Yong-Joon Cho,Kihyun Lee,Seok Hwan Yoon,Mincheol Kim,Hyunsoo Na,Sang-Cheol Park,Yoon-Seong Jeon,Jae-Hak Lee,Hana Yi,Sungho Won,Jongsik Chun +11 more
TL;DR: It is concluded that the EzTaxon-e database provides a useful taxonomic backbone for the identification of cultured and uncultured prokaryotes and offers a valuable means of communication among microbiologists who routinely encounter taxonomically novel isolates.
Journal ArticleDOI
Ribosomal DNA: molecular evolution and phylogenetic inference.
David M. Hillis,Michael T. Dixon +1 more
TL;DR: An analysis of aligned sequences of the four nuclear and two mitochondrial rRNA genes identified regions of these genes that are likely to be useful to address phylogenetic problems over a wide range of levels of divergence.
Journal ArticleDOI
The Domain-specific Probe EUB338 is Insufficient for the Detection of all Bacteria: Development and Evaluation of a more Comprehensive Probe Set
TL;DR: Two supplementary versions of probe EUB338 are designed and evaluated for in situ detection of most of those phyla not detected with this probe, which should allow a more accurate quantification of members of the domain Bacteria in future molecular ecological studies.
References
More filters
Journal ArticleDOI
Regulatory Sequences Involved in the Promotion and Termination of RNA Transcription
Martin Rosenberg,Donald L. Court +1 more
Journal ArticleDOI
Improved Estimation of Secondary Structure in Ribonucleic Acids
Ignacio Tinoco,Philip N. Borer,Barbara Dengler,Mark D. Levin,Olke C. Uhlenbeck,Donald M. Crothers,J. Bralla +6 more
TL;DR: This method can be used for predicting and assessing possible secondary structures for recently determined RNA sequences and new experimental and theoretical results allow us to improve the method, without making it more complicated.
Journal ArticleDOI
A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome
Louise Clarke,John Carbon +1 more
TL;DR: Over 80 hybrid Col El-DNA (E. coli), plasmid-bearing clones have been identified in the colony bank, and about 40 known E. coli genes have been tentatively assigned to these various plasmids.
Journal ArticleDOI
Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites.
TL;DR: A model for promoter structure and function is put forth at two independent bacteriophage T7 early promoters and the nucleotide sequence of each promoter fragment and the sequence of the mRNA coded by each fragment are presented.
Related Papers (5)
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more