Journal ArticleDOI
Experimental studies and two-dimensional modelling of a packed bed bioreactor used for production of galacto-oligosaccharides (GOS) from milk whey.
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TLDR
The prediction of substrate concentration distribution in axial and radial directions using the developed two-dimensional model equation is necessary for computing the bioreactor volume to achieve the desired GOS yield.Abstract:
In the present study, extensive experimental investigations and detailed theoretical analysis of a two-dimensional packed bed bioreactor, employed for the production of galacto-oligosaccharides (GOS) from milk whey were performed. Model equations, in one- and two-dimensions, capable of predicting the substrate concentration distribution in the bioreactor were developed by coupling mass balance equation with appropriate velocity distribution equation and solved numerically. Validation of the proposed model equations was done by a set of experimental data obtained from the bioreactor. The effects of reactor to catalyst particle diameter ratio (d t/d p), feed flowrate (10(-6)-10(-9) m(3) s(-1)), and initial lactose concentration (50-200 kg m(-3)) on substrate concentration distribution were investigated in detail. While, the distribution of substrate concentration in axial direction was independent of d t/d p, it was observed that for d t/d p <40, significant radial concentration distribution existed. It was further observed that the substrate conversion and product yield obtained experimentally showed an excellent agreement (97 ± 2 %) with the results predicted by the two-dimensional model equation, whereas, the results predicted by the one-dimensional model equation did not lie within the desired confidence level (<90 %). The results were confirmed by both curve fitting and statistical analysis. The prediction of substrate concentration distribution in axial and radial directions using the developed two-dimensional model equation is necessary for computing the bioreactor volume to achieve the desired GOS yield.read more
Citations
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Numerical methods for engineers
TL;DR: The fifth edition of "Numerical Methods for Engineers" continues its tradition of excellence and expanded breadth of engineering disciplines covered is especially evident in the problems, which now cover such areas as biotechnology and biomedical engineering.
Journal ArticleDOI
Synthesis of Galactooligosaccharides in Milk and Whey: A Review
TL;DR: This review aims to summarize the results obtained with various dairy liquids, and it rates their suitabilities to act as raw material for GOS production.
Journal ArticleDOI
Novel probiotic whey cheese with immobilized lactobacilli on casein
Dimitra Dimitrellou,Dimitra Dimitrellou,Panagiotis Kandylis,Panagiotis Kandylis,Yiannis Kourkoutas,Maria Kanellaki +5 more
TL;DR: In this article, the use of thermally-dried immobilized L.casei and L.bulgaricus on casein positively affected the production of dried whey cheeses.
Journal ArticleDOI
Recent advances in β-galactosidase and fructosyltransferase immobilization technology.
María Micaela Ureta,Gonçalo N. Martins,Onofre Figueira,Pedro Filipe Pires,Paula C. Castilho,Andrea Gómez-Zavaglia +5 more
TL;DR: The aim of this review is to go through the principal immobilization strategies addressed to achieve optimal industrial processes with special care on those reported for two types of enzymes: β-galactosidases and fructosyltransferases.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Numerical Methods for Engineers
TL;DR: Numerical methods for engineers , Numerical method for engineers, and more.
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Chemical Engineering Kinetics
TL;DR: In this paper, chemical engineering kinetics was studied in terms of kinetics in the context of Chemical Engineering kinetics and its application in the field of chemical engineering. But they did not discuss the application of the kinetics of protein kinetics.
Numerical methods for engineers
TL;DR: The fifth edition of "Numerical Methods for Engineers" continues its tradition of excellence and expanded breadth of engineering disciplines covered is especially evident in the problems, which now cover such areas as biotechnology and biomedical engineering.
Book
Numerical Methods for Chemical Engineers With Matlab Applications
A. Constantinides,Navid Mostoufi +1 more
TL;DR: This complete guide to numerical methods in chemical engineering is the first to take full advantage of MATLAB's powerful calculation environment, and enables readers to accomplish more, with less complexity, than was possible with traditional FORTRAN.
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