Journal ArticleDOI
Exploring plant tissue culture in Withania somnifera (L.) Dunal: in vitro propagation and secondary metabolite production.
TLDR
The present review provides a comprehensive report on research activities conducted in the area of tissue culture and secondary metabolite production in W. somnifera during the past years and discusses the unexplored areas which might be taken into consideration for future research.Abstract:
Withania somnifera (L.) Dunal (family: Solanaceae), commonly known as "Indian Ginseng", is a medicinally and industrially important plant of the Indian subcontinent and other warmer parts of the world. The plant has multi-use medicinal potential and has been listed among 36 important cultivated medicinal plants of India that are in high demand for trade due to its pharmaceutical uses. The medicinal importance of this plant is mainly due to the presence of different types of steroidal lactones- withanolides in the roots and leaves. Owing to low seed viability and poor germination, the conventional propagation of W. somnifera falls short to cater its commercial demands particularly for secondary metabolite production. Therefore, there is a great need to develop different biotechnological approaches through tissue and organ culture for seasonal independent production of plants in large scale which will provide sufficient raw materials of uniform quality for pharmaceutical purposes. During past years, a number of in vitro plant regeneration protocols via organogenesis and somatic embryogenesis and in vitro conservation through synthetic seed based encapsulation technology have been developed for W. somnifera. Several attempts have also been made to standardize the protocol of secondary metabolite production via tissue/organ cultures, cell suspension cultures, and Agrobacterium rhizogenes-mediated transformed hairy root cultures. Employment of plant tissue culture based techniques would provide means for rapid propagation and conservation of this plant species and also provide scope for enhanced production of different bioactive secondary metabolites. The present review provides a comprehensive report on research activities conducted in the area of tissue culture and secondary metabolite production in W. somnifera during the past years. It also discusses the unexplored areas which might be taken into consideration for future research so that the medicinal properties and the secondary metabolites produced by this plant can be exploited further for the benefit of human health in a sustainable way.read more
Citations
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Journal ArticleDOI
Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists.
Gea Guerriero,Roberto Berni,Roberto Berni,J. Armando Muñoz-Sánchez,Fabio Apone,Eslam M. Abdel-Salam,Ahmad A. Qahtan,Abdulrahman A. Alatar,Claudio Cantini,Giampiero Cai,Jean-Francois Hausman,Khawar Sohail Siddiqui,S.M. Teresa Hernández-Sotomayor,Mohammad Faisal +13 more
TL;DR: In this paper, the authors discuss the recent literature on the production of representatives of three plant secondary metabolite classes: artemisinin (a sesquiterpene), lignans (phenolic compounds) and caffeine (an alkaloid).
Journal ArticleDOI
Biotechnological Exploration of Transformed Root Culture for Value-Added Products
TL;DR: This review focuses on recent developments in hairy roots from medicinal plants, techniques to boost production of desired secondary metabolites, and the development of new technologies to study these metabolites.
Journal ArticleDOI
Metabolomics of Withania somnifera (L.) Dunal: Advances and applications.
Sarada D. Tetali,Satyabrata Acharya,Aditya B. Ankari,Vadthyavath Nanakram,Agepati S. Raghavendra +4 more
TL;DR: A critical overview of the information on the metabolomics of W. somnifera is presented and the significance of the technique for use in quality control of medicinal products is highlighted and the use of metabolomics to distinguish varieties is pointed out.
Journal ArticleDOI
Development of robust in vitro culture protocol for the propagation of genetically and phytochemically stable plants of Withania somnifera (L.) Dunal (Ashwagandha)
Kuldeep Kaur,Pritika Singh,Kulwinder Kaur,Abhishek Bhandawat,Panchsheela Nogia,Pratap Kumar Pati +5 more
TL;DR: For prolonged maintenance of in vitro shoots, a multi-step protocol involving the rotational subculture of Withania shoots in the auxin-cytokinin containing culture medium has been developed to minimize the adverse effects of cytokinin.
Journal ArticleDOI
Ashwagandha: Advances in plant biotechnological approaches for propagation and production of bioactive compounds.
Ajay G. Namdeo,Deepa K. Ingawale +1 more
TL;DR: This review underlines the advances in plant biotechnological approaches for the propagation of W. somnifera and production of its bioactive compounds and highlights a variety of bioteschnological strategies both for prompt propagation andproduction of different bioactive secondary metabolites.
References
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