Extracellular Electrical Fields Direct Wound Healing and Regeneration
Mark A. Messerli,David M. Graham +1 more
TLDR
The generation of endogenous EFs, the results of their alteration, and the mechanisms by which cells may sense these weak fields are reviewed to enable current and future therapeutic applications to be optimized.Abstract:
Endogenous DC electric fields (EFs) are important, fundamental components of development, regeneration, and wound healing. The fields are the result of polarized ion transport and current flow through electrically conductive pathways. Nullification of endogenous EFs with pharmacological agents or applied EFs of opposite polarity disturbs the aforementioned processes, while enhancement increases the rate of wound closure and the extent of regeneration. EFs are applied to humans in the clinic, to provide an overwhelming signal for the enhancement of healing of chronic wounds. Although clinical trials, spanning a course of decades, have shown that applied EFs enhance healing of chronic wounds, the mechanisms by which cells sense and respond to these weak cues remains unknown. EFs are thought to influence many different processes in vivo. However, under more rigorously controlled conditions in vitro, applied EFs induce cellular polarity and direct migration and outgrowth. Here we review the generation of endogenous EFs, the results of their alteration, and the mechanisms by which cells may sense these weak fields. Understanding the mechanisms by which native and applied EFs direct development and repair will enable current and future therapeutic applications to be optimized.read more
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References
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Journal ArticleDOI
Controlling Cell Behavior Electrically: Current Views and Future Potential
TL;DR: This review aims to resolve issues by describing the historical context of bioelectricity, the fundamental principles of physics and physiology responsible for DC electric fields within cells and tissues, the cellular mechanisms for the effects of small electric fields on cell behavior, and the clinical potential for electric field treatment of damaged tissues.
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Electrical signals control wound healing through phosphatidylinositol-3-OH kinase-γ and PTEN
Min Zhao,Bing Song,Jin Pu,Teiji Wada,Brian Reid,Guangping Tai,Fei Wang,Fei Wang,Aihua Guo,Petr Walczysko,Yu Gu,Takehiko Sasaki,Akira Suzuki,John V. Forrester,Henry R. Bourne,Peter N. Devreotes,Colin D. McCaig,Josef M. Penninger +17 more
TL;DR: It is shown that electric fields, of a strength equal to those detected endogenously, direct cell migration during wound healing as a prime directional cue.
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Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor
Yoshimichi Murata,Hirohide Iwasaki,Hirohide Iwasaki,Mari Sasaki,Mari Sasaki,Kazuo Inaba,Yasushi Okamura +6 more
TL;DR: In this paper, a voltage-sensor-containing phosphatase (Ci-VSP) was proposed, which displays channel-like gating currents and directly translates changes in membrane potential into the turnover of phosphoinositides.
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Calcium flickers steer cell migration
TL;DR: Findings show how the exquisite spatiotemporal organization of calcium microdomains can orchestrate complex cellular processes such as cell migration.
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Electrical fields in wound healing-An overriding signal that directs cell migration.
TL;DR: Clinically, it is highly desirable to develop practical and reliable technologies for wound healing management exploiting the electric signaling, and genetic manipulation of PI3 kinase/Pten and integrin beta4 demonstrated the importance of those molecules.