Journal ArticleDOI
Facilitating In Situ Tumor Imaging with a Tetrahedral DNA Framework‐Enhanced Hybridization Chain Reaction Probe
TLDR
The T‐probe system efficiently and accurately images tumor‐related biomarkers in vitro and in vivo, thereby demonstrating great potential for clinical diagnosis and therapeutic applications.Abstract:
Rapid and efficient tools for early cancer detection have diagnostic and therapeutic value. Given that the DNA hairpin‐based hybridization chain reaction (HCR) is effective in detecting various biological targets, a tetrahedral framework DNA‐enhanced (TDN‐enhanced) HCR detection system (T‐probe system) is introduced for cancer‐related targets and its versatility is demonstrated by detecting intracellular target miRNA 21 and cellular membrane target nucleolin. Benefiting from the spatial confinement of the TDN, the T‐probe system demonstrates a high detection rate. It increases the reaction efficiency of nude hairpins in vitro while accurately and rapidly recognizing both membrane and intracellular cancer‐related targets in living cells. Furthermore, it exhibits superior fluorescence in vivo within 15 s of peripheral‐tumor injection and 10 min of tail‐vein injection. The T‐probe system efficiently and accurately images tumor‐related biomarkers in vitro and in vivo, thereby demonstrating great potential for clinical diagnosis and therapeutic applications.read more
Citations
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Journal ArticleDOI
Modulation of the Crosstalk between Schwann Cells and Macrophages for Nerve Regeneration: A Therapeutic Strategy Based on a Multifunctional Tetrahedral Framework Nucleic Acids System
TL;DR: Results demonstrated that MiDs could amplify the ability of SCs to recruit macrophages and facilitate their polarization into the pro-healing M2 phenotype to reconstruct the post-injury microenvironment and could initiate the adaptive intracellular reprogramming ofSCs within a short period to further promote axon regeneration and remyelination.
Journal ArticleDOI
A Lysosome‐Activated Tetrahedral Nanobox for Encapsulated siRNA Delivery
Yang Gao,Xingyu Chen,Taoran Tian,Tao Zhang,Shaojingya Gao,Xiaolin Zhang,Yang Yao,Yunfeng Lin,Xiaoxiao Cai +8 more
TL;DR: Inspired by the original stable space inside the tetrahedral scaffold, a dynamic lysosome-activated tFNA nanobox is fabricated for completely encapsulating a short interfering RNA (siRNA) of interest and an excellent silencing effect on the target TNFα gene is achieved.
Journal ArticleDOI
Prospects and challenges of dynamic DNA nanostructures in biomedical applications
TL;DR: In this article , the authors introduce the recent efforts and discoveries in DNA nanotechnology, highlighting the uses of dynamic DNA nanostructures in biomedical applications, including basic research, active drug delivery, biosensor development, and tissue engineering.
Journal ArticleDOI
DNA Walkers for Biosensing Development
TL;DR: The ability to design nanostructures with arbitrary shapes and controllable motions has made DNA nanomaterials used widely to construct diverse nanomachines with various structures and functions, including programmability, stability, biocompatibility, and can be modified with different functional groups.
Journal ArticleDOI
Antiepilepticus Effects of Tetrahedral Framework Nucleic Acid via Inhibition of Gliosis-Induced Downregulation of Glutamine Synthetase and Increased AMPAR Internalization in the Postsynaptic Membrane.
Jianwei Zhu,Yuting Yang,Wenjuan Ma,Yangyang Wang,Lihua Chen,Huan Gui Xiong,Cheng Yin,Zongze He,Wei Yun Fu,Ruxiang Xu,Yunfeng Lin +10 more
TL;DR: tFNA, an assembled nucleic acid nanoparticle, showed an excellent ability to the cross blood-brain barrier (BBB) to inhibit M1 microglial activation and A1 reactive astrogliosis in the hippocampus of mice after status epilepticus and inhibited the downregulation of glutamine synthetase.
References
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Journal ArticleDOI
Triggered amplification by hybridization chain reaction.
Robert M. Dirks,Niles A. Pierce +1 more
TL;DR: This work introduces the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment, which allows DNA to act as an amplifying transducer for biosensing applications.
Journal ArticleDOI
Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication.
Russell P. Goodman,Iwan A. T. Schaap,Iwan A. T. Schaap,C. F. Tardin,C. F. Tardin,Christoph M. Erben,Christoph M. Erben,Richard M. Berry,Richard M. Berry,Christoph F. Schmidt,Christoph F. Schmidt,Andrew J. Turberfield,Andrew J. Turberfield +12 more
TL;DR: A family of DNA tetrahedra, less than 10 nanometers on a side, that can self-assemble in seconds with near-quantitative yield of one diastereomer are reported that can be connected by programmable DNA linkers.
Journal ArticleDOI
In vivo magnetic resonance imaging of transgene expression.
Ralph Weissleder,Anna Moore,Umar Mahmood,Rajeev Bhorade,Helene Benveniste,E. Antonio Chiocca,James P. Basilion +6 more
Journal ArticleDOI
Quantifying colocalization by correlation: the Pearson correlation coefficient is superior to the Mander's overlap coefficient.
Jeremy Adler,Ingela Parmryd +1 more
TL;DR: The MOC is a confusing hybrid measurement that combines correlation with a heavily weighted form of co‐occurrence, favors high intensity combinations, downplays combinations in which either or both intensities are low and ignores blank pixels, and is not suitable for making measurements of colocalization either by correlation or co-occurrence.
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Self-Assembled Multivalent DNA Nanostructures for Noninvasive Intracellular Delivery of Immunostimulatory CpG Oligonucleotides
TL;DR: Functional, multivalent DNA nanostructures are developed by appending unmethylated CpG motifs to three-dimensional DNA tetrahedra that are compact, mechanically stable, and noncytotoxic and expected to become a promising tool for targeted drug delivery.