Journal ArticleDOI
Galactose inhibition of citric acid production from glucose by Aspergillus niger
TLDR
Galactose interfered with the glucose-repression of the key enzyme 2-oxoglutarate dehydrogenase, and caused inhibition of citric acid production, and also reduced the rate of glucose utilization.Abstract:
Previous work in this laboratory has demonstrated that although Aspergillus niger can readily utilize galactose, no citric acid is produced from this carbon source (Hossain et al 1984) Experiments were now conducted where galactose was added at various concentrations to synthetic growth medium containing glucose as carbon source, so that the effect of galactose on citric acid production from glucose could be observed The results showed that the presence of galactose or a product of galactose metabolism caused inhibition of citric acid production, and also reduced the rate of glucose utilization Enzyme analyses using mycelial cell-free extracts indicated that galactose interfered with the glucose-repression of the key enzyme 2-oxoglutarate dehydrogenaseread more
Citations
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A Simplified Method
TL;DR: A simplified method for the determination of the rate of synthesis of albumin after the injection of 14C-carbonate is described in this article, where good agreement is obtained between the specific activity of urinary urea, measured by means of our method and by the manometric method.
Journal ArticleDOI
Utilization of different agro-industrial wastes for sustainable bioproduction of citric acid by Aspergillus niger
TL;DR: In this paper, the potential of different agro-industrial wastes for hyper production of citric acid through solid-state and submerged fermentation by Aspergillus niger NRRL 567 and NRRL 2001 was evaluated.
Journal ArticleDOI
Citric acid production from whey with sugars and additives by Aspergillus niger
TL;DR: In this article, Aspergillus niger produces citric acid from whey with different concentrations of sucrose, glucose, fructose, galactose, riboflavin, tricalcium phosphate and methanol.
Journal ArticleDOI
Citric acid production in a bubble-column reactor using cells of the yeast Candida guilliermondii immobilized by adsorption onto sawdust
TL;DR: In comparison with literature reports describing other cell immobilization techniques, adsorption onto sawdust allows similar reactor productivities while being cheap and permitting simple immobilization and reactor operation.
Journal ArticleDOI
Citric Acid Production by Aspergillus niger Cultivated on Parkia biglobosa Fruit Pulp
TL;DR: The studies revealed that production parameters (pH, inoculum size, substrate concentration, incubation temperature, and fermentation period) had profound effect on the amount of citric acid produced.
References
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Journal ArticleDOI
A simplified method for the quantitative assay of small amounts of protein in biologic material.
Book ChapterDOI
[12] α-ketoglutarate dehydrogenase complex from Escherichia coli
TL;DR: The ferricyanide reduction assay described provides an estimate of the activity of the α-ketoglutarate dehydrogenase component (E1) of the complex, the only reliable assay for the intact complex however it is not suitable for crude preparations that contain DPNH oxidase.
A Simplified Method
TL;DR: A simplified method for the determination of the rate of synthesis of albumin after the injection of 14C-carbonate is described in this article, where good agreement is obtained between the specific activity of urinary urea, measured by means of our method and by the manometric method.
Journal ArticleDOI
The effect of the sugar source on citric acid production by Aspergillus niger
TL;DR: The activity of aconitase showed a direct relationship to the citric acid production rate, and the activity was highest when sucrose was the sugar source, and lowest when galactose was the source.
Journal ArticleDOI
Pyruvate carboxylase of Aspergillus niger: kinetic study of a biotin-containg carboxylase
Helen A. Feir,Isamu Suzuki +1 more
TL;DR: Pyruvate carboxylase was partially purified from Aspergillus niger and the properties were studied, and the enzyme was shown to be a biotin-containing enzyme by its in activation by avidin and protection against such inactivation by biotin.
Related Papers (5)
The effect of the sugar source on citric acid production by Aspergillus niger
The effect of methanol on citric acid production from galactose by Aspergillus niger
Production of citric acid from whey permeate by fermentation using Aspergillus niger
M. Hossain,John D. Brooks +1 more