Gangliosides of human, cat, and rabbit spinal cords and cord myelin.
K Ueno,Susumu Ando,Robert K. Yu +2 more
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TLDR
Gangliosides were isolated from whole spinal cords and cord myelin of human, cat, and rabbit by a revised methodology and the most prominent and consistent features were the reduced concentration of Gd1a and increased amounts of Gm3 and Gd3.About:
This article is published in Journal of Lipid Research.The article was published on 1978-09-01 and is currently open access. It has received 204 citations till now. The article focuses on the topics: Myelin & Ganglioside.read more
Citations
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Journal ArticleDOI
A procedure for the quantitative isolation of brain gangliosides.
Lars Svennerholm,Pam Fredman +1 more
TL;DR: The yield of gangliosides obtained by extraction of the tissue twice with twenty volumes of chloroform/methanol/water was larger than that obtained with all other solvents tested, including tetrahydrofuran/phosphate buffer.
Book ChapterDOI
Gangliosides: structure, isolation, and analysis.
Robert W. Ledeen,Robert K. Yu +1 more
TL;DR: This chapter discusses structure and procedure for isolation, and analysis of gangliosides, along with a gas-liquid chromatographic method that has the attributes, of specificity and sensitivity.
Journal ArticleDOI
Lipids of nervous tissue: composition and metabolism.
TL;DR: The period of discovery of new lipids in the nervous system appears to be over and all the major lipid components have been discovered and a great deal is now known about their structure and metabolism.
Book ChapterDOI
Isolation and Characterization of Myelin
TL;DR: This chapter will discuss isolation procedures and the composition of purified myelin obtained by these procedures, and the techniques developed for isolating myelin membranes essentially free of other subcellular structures.
Journal ArticleDOI
The Use of Sep‐Pak™ C18 Cartridges During the Isolation of Gangliosides
TL;DR: The use of Sep‐Pak™ C18 reverse‐phase cartridges to adsorb gangliosides from aqueous solutions was studied and retained lipids can be eluted with methanol or chloroform‐methanol.
References
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Journal ArticleDOI
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
TL;DR: A new method for the quantitive determination of sialic acids is described, which is about 50% more sensitive than the orcinol-hydrochloric acid method generally used and considerably lower with the resorcin reagent.
Journal ArticleDOI
CHROMATOGRAPHlC SEPARATION OF HUMAN BRAIN GANGLIOSIDES
TL;DR: Chromatographic methods for the quantitative isolation and separation of human brain gangliosides are described and it is shown that predominantly the gangLiosides have a common basic structure : N-acyl-sphingosine-glucose-Galactose-galactoseN-acetylgalactosamine-gal actose to which one or more molecules of N-acetelneuraininic acid are bound.
Journal ArticleDOI
Myelination in rat brain: method of myelin isolation.
TL;DR: The myelin prepared from 20‐day animals had the highest content of the first three constituents and the values of the four constituents were relatively constant per unit weight of myelin; the amounts of nucleic acid and ganglioside recovered in the myelin fractions increased with increasing age and myelin yield.
Journal ArticleDOI
The determination of sugar in blood and spinal fluid with anthrone reagent
TL;DR: The authors' procedure for the determination of dextran to the estimation of the sugar in blood and spinal fluid is adapted, and a stabilized anthrone reagent has been developed, and certain findings of interest are reported.
Journal ArticleDOI
The Sialic Acids XI. A PERIODATE-RESORCINOL METHOD FOR THE QUANTITATIVE ESTIMATION OF FREE SIALIC ACIDS AND THEIR GLYCOSIDES
TL;DR: The periodate-resorcinol method was substantially more sensitive than the resorcinl procedure, was not affected by lipids, amino acids, or sugars, and could be used to detect free or glycosidically bound sialic acids on paper chromatograms.