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Open AccessJournal ArticleDOI

Genetic characterization of Egyptian buffalo CSN3 gene

Soheir M. El Nahas, +2 more
- 01 Dec 2013 - 
- Vol. 11, Iss: 2, pp 123-127
TLDR
The reason why buffalo samples, analyzed by RFLP technique, using HindIII and HinfI used in cattle, were mistakenly identified as BB monomorphic isInvestigation of genetic polymorphism k-casein gene investigated in lactating Egyptian buf- falo shows that Egyptian buffalo has both alleles A and B with allelic frequencies of 0.57.
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This article is published in Journal of Genetic Engineering and Biotechnology.The article was published on 2013-12-01 and is currently open access. It has received 11 citations till now. The article focuses on the topics: Open reading frame & Silent mutation.

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Citations
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Journal ArticleDOI

Genetic association between SNPs in the DGAT1 gene and milk production traits in Murrah buffaloes

TL;DR: Polymorphisms in the DGAT1 gene in Murrah buffaloes identified and investigated the associations to milk production and quality traits indicate that assisted marker selection might be done with considerations to balance production and udder health.
Proceedings ArticleDOI

Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

TL;DR: The result showed that BB genotypes were found at the locus of CSN3 gene in all samples, which suggests that genotyping using HindIII reveals monomorphics of BB types accociated with milk content of casein.

Genotyping of the kappa-casein and leptin genes in Cuban water buffalo by PCR-RFLP

TL;DR: Genetic analysis of loci affecting production and reproduction traits in livestock animals make possible the evaluation of animal breeding values using molecular markers and a higher number of animals and SNPs in the leptin and/or other genes should be analyzed for a more accurate estimate of the genetic diversity in this population.
Journal ArticleDOI

AcuI identifies water buffalo CSN3 genotypes by RFLP analysis

TL;DR: Using sequence analysis, three buffalo-CSN3 genotypes were identified and using in silico analysis, the restriction enzyme AcuI was suggested for buffalo- CSN3genotyping and it is shown experimentally thatAcuI restriction enzyme is useful in genotyping buffalo- CSI3 gene by RFLP analysis.
Journal ArticleDOI

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

TL;DR: Results revealed a monomorphic pattern of Egyptian buffaloes and genotyped as “AA” animals which signified that PCR-RFLP assay with HaeIII enzyme for β-LG gene failed to discover any evidence of polymorphism in Egyptian buffalo under the circumstances of this study or all studied animals possess only one allele.
References
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Journal ArticleDOI

A simple salting out procedure for extracting DNA from human nucleated cells

TL;DR: A rapid, safe and inexpensive method was developed to simplify the deprotein-ization procedure that yielded quantities comparable to those obtained from phenol-chloroform extractions, rendering the entire process of RFLP analysis free of toxic materials.
Journal ArticleDOI

Structure and Function of Milk Protein Genes

TL;DR: Better knowledge of both the structure and function of milk protein genes, which has already allowed the use of powerful techniques for the rapid identification of alleles, offers the potential for the genetic modification of milk composition.
Journal ArticleDOI

The Water Buffalo: A Review

TL;DR: The water buffalo has been largely neglected by science but, in the course of the past few decades, there has been growing interest in the improvement of national stocks by selective breeding and improved husbandry.
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