Journal ArticleDOI
Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs
TLDR
UPGMA cluster analysis carried out on these data separately for RAPDs and AFLPs and on the combined data reflected, to some extent, pedigree relationships and cophenetic correlations that indicate a good fit of respective clusters to genetic similarity data.Abstract:
In order to obtain an overview of the genetic diversity present within the set of pea cultivars released in Germany, 21 cultivars were analysed at the DNA level by random amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphisms (AFLPs), as well as for agronomic traits. Yield of grain cultivars ranged from 2.95 to 3.87 t/ha. Based on the screening of 60 RAPD primers and 32 Eco RI + 3/Mse I+3 AFLP primer combinations, 20 RAPD primers and 11 Eco RI + 3/MseI+ 3 primer combinations generating polymorphic and distinct fragments were chosen for estimation of genetic diversity. Twenty RAPD primers amplified a total of 314 scorable bands ranging from about 262 bp to 1996 bp. Of these, 175 fragments (55.7%) were polymorphic. Based on these data, genetic similarity (GS) was estimated between 0.80 (‘Lisa’ vs.‘Grapis’) and 0.94 (‘Bohatyr’ vs. ‘Sponsor’; mean GS = 0.88). Eleven AFLP primer combinations led to the amplification of 949 scorable fragments ranging from 43 to 805 bp and of these, 462 (48.7%) were polymorphic. Genetic similarity based on AFLPs was calculated between 0.85 (‘Lisa’ vs.‘Laser’) and 0.94 (‘Bohatyr’ vs. ‘Sponsor’, mean GS = 0.90). Correlation of genetic similarity estimated on RAPDs and AFLPs was estimated at r = 0.79** using Spearman's rank correlation coefficient and at r = 0.84 by the Mantel test, respectively. UPGMA cluster analysis carried out on these data separately for RAPDs and AFLPs and on the combined data reflected, to some extent, pedigree relationships and cophenetic correlations (r = 0.89 for RAPDs, r = 0.88 for AFLPs, and r = 0.93 RAPDs + AFLPs) indicate a good fit of respective clusters to genetic similarity data. The correlation of cluster analyses to pedigree information and the impact on parental genotype selection is discussed.read more
Citations
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Journal ArticleDOI
A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers
J. Souframanien,T. Gopalakrishna +1 more
TL;DR: Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to study the DNA polymorphism in elite blackgram genotypes, and the pattern of clustering of the genotypes remained more or less the same in ISSR and combined data of RAPD.
Journal ArticleDOI
Genetic diversity among varieties and wild species accessions of pea (Pisum sativum L.) based on molecular markers, and morphological and physiological characters.
TL;DR: A number of variety-specific markers were identified in the current study, which could be useful for variety identification and Breeding strategies to maintain or enhance the genetic diversity of future varieties are proposed.
Journal ArticleDOI
Towards marker-assisted selection in pulses: a review
TL;DR: The progress made, limitations encountered and future possibilities for the application of marker-assisted selection in the genetic improvement of pulse crops are reviewed.
Journal ArticleDOI
Genetic diversity within Pisum sativum using protein- and PCR-based markers
Alain Baranger,Grégoire Aubert,G. Arnau,A. L. Lainé,G. Deniot,J. Potier,C. Weinachter,Isabelle Lejeune-Hénaut,J. Lallemand,Judith Burstin +9 more
TL;DR: This molecular marker-based classification allowed us to trace back major lineages of pea breeding in Western Europe over the last decades, and to follow the main breeding objectives: increase of seed weight, introduction of the afila foliage type and white flowers, and improvement of frost tolerance for winter-sown peas.
Journal ArticleDOI
Comparative analysis on the genetic relatedness of Sorghum bicolor accessions from Southern Africa by RAPDs, AFLPs and SSRs
TL;DR: The coefficient of variation of the estimated genetic similarity decreased with an increasing number of bands and was lowest using AFLPs, while SSR, Δμ-SSR and RAPD-based similarity estimates had low mean bootstrap probabilities (24%, 27%, 30%, respectively).
References
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Journal ArticleDOI
AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal ArticleDOI
Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs
Ivan Pejić,Paolo Ajmone-Marsan,Michele Morgante,V. Kozumplick,P. Castiglioni,G. Taramino,M. Motto +6 more
TL;DR: In this article, a comparison of DNA-based fingerprinting techniques, including RAPD, SSR, AFLP and AFLP, was performed for maize inbred lines and the results showed that AFLPs were the most efficient marker system because of their capacity to reveal several bands in a single amplification.
Journal ArticleDOI
Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs
Joanne Russell,John Fuller,Malcolm Macaulay,B. G. Hatz,Ahmed Jahoor,Wayne Powell,Robbie Waugh +6 more
TL;DR: RFLPs, AFLPs, RAPDs and SSRs were used to determine the genetic relationships among 18 cultivated barley accessions and the results compared to pedigree relationships where these were available and the choice of appropriate technology for different aspects of germplasm evaluation is discussed.
Journal ArticleDOI
Genetic distribution of Bare-1-like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP)
Robbie Waugh,Karen McLean,Andrew J. Flavell,Stephen R. Pearce,Amar Kumar,Bill Thomas,Wayne Powell +6 more
TL;DR: A polymerase chain reaction (PCR)-based method which exploits this polymorphism for the generation of molecular markers in barley, which produces amplified fragments containing a Bare–1-like retrotransposon long terminal repeat (LTR) sequence at one end and a flanking host restriction site at the other.