Journal ArticleDOI
Genetically engineered anthocyanin pathway for high health-promoting pigment production in eggplant
TLDR
In this paper, the authors introduced SmMYB1 into a non-anthocyanin-accumulating eggplant cultivar (Solanum aethiopicum group Gilo) via Agrobacterium-mediated transformation.Abstract:
Anthocyanins, natural pigments with high antioxidant activities, are widely distributed in the plant kingdom and play important roles in various physiological processes. Much effort has been committed to enhancing the anthocyanin content of these health-promoting pigments in vegetables and grains, for their eye-catching colors and special nutrients. Previously, we reported that the SmMYB1 gene encoding a R2R3 MYB transcription factor participated in the regulation of anthocyanin biosynthesis in the peel of eggplant. In this work, we introduced SmMYB1 into a non-anthocyanin-accumulating eggplant cultivar (Solanum aethiopicum group Gilo) via Agrobacterium-mediated transformation. Genetically engineered plants exhibited high concentrations of anthocyanin in leaves, petals, stamens, and fruit peels under normal growth conditions, especially in fruit flesh. Furthermore, highly methylated anthocyanins, malvidin 3-(p-coumaroyl)rhamnoside(glucoside)-5-glucoside and malvidin 3-(feruloyl)rhamnoside(glucoside)-5-glucoside, were separated from the purple fruit flesh and identified by HPLC–ESI–MS/MS. qRT-PCR analysis revealed that most anthocyanin structural genes were dramatically up-regulated in the tissues of transgenic lines compared with non-transformed plants. In addition, the transgenic seedlings had greater tolerance to freezing stress and better recovery under rewarming conditions. These results provide a good foundation for the breeding of new eggplant cultivars with more healthy agronomic traits in future studies.read more
Citations
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Journal ArticleDOI
Roles of R2R3-MYB transcription factors in transcriptional regulation of anthocyanin biosynthesis in horticultural plants
Aung Htay Naing,Chang Kil Kim +1 more
TL;DR: Which R2R3-MYB transcription factors control the transcriptional regulation of anthocyanin structural genes involved in the specific branches of the anthocianin biosynthetic pathway in various horticultural plants are described.
Journal ArticleDOI
Understanding the genetic regulation of anthocyanin biosynthesis in plants - Tools for breeding purple varieties of fruits and vegetables.
Samuel Chaves-Silva,Adolfo Luís dos Santos,Antonio Chalfun-Junior,Jian Zhao,Lázaro Eustáquio Pereira Peres,Vagner A. Benedito +5 more
TL;DR: The current literature on the anthocyanin biosynthesis pathway in model and crop species is reviewed, including the structural and regulatory genes involved in the differential pigmentation patterns of plant structures, in order to create more efficient breeding strategies to boost the biosynthesis and accumulation of Anthocyanins in fresh fruits and vegetables.
Journal ArticleDOI
Oxidative Stress Associated with Chilling Injury in Immature Fruit: Postharvest Technological and Biotechnological Solutions.
Juan Luis Valenzuela,Susana Manzano,Francisco Palma,F. Carvajal,Dolores Garrido,Manuel Jamilena +5 more
TL;DR: The physiological and biochemical factors that are associated with the oxidative stress and the development of CI symptoms in these commodities are defined and the different physical, chemical and biotechnological approaches that have been proposed to reduce oxidative stress while enhancing the chilling tolerance of vegetable fruits are discussed.
Journal ArticleDOI
Advances in biochemical mechanisms and control technologies to treat chilling injury in postharvest fruits and vegetables
TL;DR: In this paper, a review summarizes the temperature and symptoms of chilling injury in common post-harvest fruits and vegetables, and the potential biochemical mechanisms of different postharvest technologies are also attributed and attributed to the following strategies: maintaining membrane structure and sufficient energy supply, enhancing the antioxidant system, promoting the arginine pathway, regulating sugar metabolism, and activating the C-repeat/dehydration response element binding factor (CBF) genes.
Journal ArticleDOI
Looking at Flavonoid Biodiversity in Horticultural Crops: A Colored Mine with Nutritional Benefits.
TL;DR: The role of flavonoid biodiversity among the major horticultural species, which is responsible of differences among closely related species and influences the qualitative/quantitative composition, is reviewed.
References
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TL;DR: Some of the recent advances in flavonoid research are reviewed and the role of anthocyanins and flavones in providing stable blue flower colours in the angiosperms is outlined.
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TL;DR: Three classes of pigments act as visible signals to attract insects, birds and animals for pollination and seed dispersal, and protect plants from damage caused by UV and visible light.
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Genetics and Biochemistry of Anthocyanin Biosynthesis
TL;DR: The characterization of genetically defined mutations has enabled the order of many reactions in anthocyanin synthesis and their modification to be elucidated, and the more recent developments in gene isolation and characterization are concentrated.
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Flavonoids: a colorful model for the regulation and evolution of biochemical pathways
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