Glucose induced inactivation of malate dehydrogenase in intact yeast cells.
W. Duntze,D. Neumann,H. Holzer +2 more
TLDR
Evidence is provided that a metabolite formed in the first steps of glycolysis might be responsible for initiation of malate dehydrogenase inactivation, which is initiated by the addition of glucose or related hexoses to the medium.Abstract:
The glucose-dependent inactivation of malate dehydrogenase (l-malate: NAD oxidoreductase, EC 1.1.1.37) in acetate-grown yeast cells was studied in vivo. Among different yeast species inactivation was observed only in strains of the genus Saccharomyces. The phenomenon is initiated by the addition of glucose or related hexoses to the medium and is reversibly interrupted at 0°. By use of a yeast mutant requiring tryptophan, it was shown that malate dehydrogenase inactivation is not influenced by inhibition of protein synthesis, whereas recovery of enzyme activity in inactivated cells requires the presence of an energy source and tryptophan and is presumably due to de novo protein synthesis. The reported results provide evidence that a metabolite formed in the first steps of glycolysis might be responsible for initiation of malate dehydrogenase inactivation.read more
Citations
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Journal ArticleDOI
Regulation of trehalose mobilization in fungi.
TL;DR: The inhibition of regulatory trehalase by nitrogen sources in the presence of glucose and the induction of fungal spore germination by heat shock are studied.
Journal ArticleDOI
Catabolite inactivation in yeast
TL;DR: This "catabolite inactivation" in yeast is described and discussed and it is shown that glucose and its catabolites not only cause repression of enzyme synthesis in some organisms but also inactivation of certain enzymes.
Journal ArticleDOI
Yeast Sugar Transporters
TL;DR: The molecular analysis of glucose transporters in Saccharomyces has revealed the existence of a multigene family of sugar carriers, raising the question of the actual role of all of these proteins in sugar catabolism.
Book ChapterDOI
The Significance of Peroxisomes in the Metabolism of One-Carbon Compounds in Yeasts
TL;DR: The information on various aspects of the role of peroxisomes in the metabolism of one-carbon compounds in yeasts clearly shows that these unicellular organisms offer an almost ideal model system for the study of function, morphogenesis, and turnover of these intriguing organelles.
Journal ArticleDOI
Glycolysis mutants in Saccharomyces cerevisiae.
TL;DR: Mutants have been isolated in S. cerevisiae with the phenotype of growth on pyruvate but not on glucose, or growth on rich medium with pyruVate but inhibition by glucose, and double mutants now also lacking hexokinase, phosphofructokinase or several enzymes of glycolysis.
References
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Journal ArticleDOI
Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources.
ES Polakis,W. Bartley +1 more
TL;DR: Glucose was much more effective in inducing the anaerobic pathways than was galactose and the 2-oxoglutarate-oxidase system was found to be the least active enzyme of the citric acid cycle.
Journal ArticleDOI
Repression von alkoholdehydrogenase, malatdehydrogenase, isocitratlyase und malatsynthase in hefe durch glucose
TL;DR: It is assumed that repression of the synthesis of alcohol dehydrogenase, malate dehydrogen enzyme, isocitrate lyase and malate synthase is not caused by metabolites but by glucose itself, glucose 6-phos-phate, or perhaps by changes in the concentrations of adenosine 5'-triphosphate or -diphosphate.
Journal ArticleDOI
Biochemical correlates of respiratory deficiency: VII. Glucose repression
TL;DR: A model is presented which postulates that, during repression there takes place, at least in part, a disassembly of functional mitochondria to nonfunctional, probably particulate, entities and that newfunctional mitochondria arise from them during de-repression.
Journal ArticleDOI
Yeast Malate Dehydrogenase: Enzyme Inactivation in Catabolite Repression
J. J. Ferguson,M. Boll,H. Holzer +2 more
TL;DR: Kinetic evidence suggests that malate dehydrogenase from acetate-induced and glucose-repressed yeast are different molecular species, and possible mechanisms for “inactivation-repression” are discussed.
Journal ArticleDOI
Isoenzyme der malatdehydrogenase und ihre regulation in Saccharomyces cerevisiae
TL;DR: It is concluded that a repression of c-malate dehydrogenase synthesis by glucose occurs and this regulating mechanism is useful for the cell, because in the glycoxylate cycle c- malate dehydration participates in the gluconeogenesis from acetate or ethanol.
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Yeast Malate Dehydrogenase: Enzyme Inactivation in Catabolite Repression
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