Open AccessJournal Article
Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro.
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TLDR
Melanolipofuscin is a unique marker for nondividing cells and may facilitate studies of the age-dependent loss of replicability of human RPE.Abstract:
The in vitro growth characteristics and morphology of human retinal pigment epithelium (RPE) cells from adult donor eyes (15 to 100 years of age) have been studied. Although RPE cells are viable in culture for several months, only a fraction of the cells actually divide. Rapid cell proliferation and confluency of a culture occur from 10 to 30 days after seeding. The time for confluency is both age- and media-dependent; the number of cells that are potential dividers and contribute to confluency decreases with increasing donor age. Since melanolipofuscin granules do not form in vitro and are diluted by cell division, stationary (nondividing) cells can be distinguished from the dividing cells by the presence of dense clusters of melanolipofuscin granules in the stationary cells. Confluent cultures contain a monolayer of relatively clear polygonal cells with densely pigmented stationary cells scattered throughout. Stationary cells can often represent as much as 95% of the original RPE cell population. Ultrastructurally all cells appear epithelioid, with apical-basal polarity, junctional complexes, and cytoplasmic organization characteristic of RPE cells in vivo. Stationary cells are extremely large, with melanolipofuscin granules clustered around the nucleus. Melanolipofuscin is a unique marker for nondividing cells and may facilitate studies of the age-dependent loss of replicability of human RPE.read more
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References
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Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Cellular senescence and DNA synthesis. Thymidine incorporation as a measure of population age in human diploid cells.
TL;DR: Using 10% class intervals for the incidence of labelled nuclei, it is possible to distinguish the ‘age’ of populations which differ by about 5 passages or less and evaluate simultaneously and in a reproducible way the current age of the culture, as well as the remaining proliferative capacity of the population.
Journal Article
Lipofuscin and melanin of human retinal pigment epithelium. Fluorescence, enzyme cytochemical, and ultrastructural studies.
TL;DR: The studies indicate that a dynamic, complex interrelationship exists between the various components of the phagolysosomal system and the melanin granules in the RPE cytoplasm and suggests that it undergoes modification or degradation there.
Journal ArticleDOI
Defective phagocytosis of isolated rod outer segments by RCS rat retinal pigment epithelium in culture
RB Edwards,RB Szamier +1 more
TL;DR: It is demonstrated that RCS rat pigment epithelial cells contain a defect in the mechanism for phagocytizing outer segments, which causes them to rarely ingests outer segment material.
Journal Article
Electron microscopic observations of human retinitis pigmentosa, dominantly inherited.
Helga Kolb,Peter Gouras +1 more
TL;DR: Formalin-fixed eyes of a patient with autosomally dominant retinitis pigmentosa were studied by light and electron microscopy and it was found that foveal pigment epithelium cells contained excessive amounts of lipofusdn in large spherical clusters, reduced amounts of melanin, and were in different stages of migration away from Bruch's membrane.
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