Growth Promotion and Induction of Systemic Resistance in Rice Cultivar Co-47 (Oryza sativa L.) by Methylobacterium spp.
Munusamy Madhaiyan,Selvaraj Poonguzhali,M. Senthilkumar,Sundaram Seshardi,Hee-Kyung Chung,Jinchul Yang,Subbiah Sundaram,Tongmin Sa +7 more
TLDR
The results suggest that Methylobacterium inoculation may alter rice susceptibility to R. solani and emphasizes the importance of evaluating induced systemic resistance while studying plant-associated growth promoting bacteria.Abstract:
Pink-pigmented facultatively methylotrophic bacteria (PPFMs), persistent colonizers of plant leaf surfaces, belong to the genus Methrlobacterium and are mostly transmitted through seeds. Plant growth-promoting activity of methylotrophic bacteria and their effects on disease suppression were evaluated on rice under greenhouse conditions. Rice seeds were inoculated with Methylobacterium sp. strain PPFM-Os-07 and seed germination was evaluated in terms of morphometric measurements, seedling growth, rate of germination (R(subscript G)), and seedling vigour index (SVI). Another experiment was carried out to study the induction of pathogenesis-related proteins (PR-proteins) in rice plants that were inoculated with methylotrophic bacteria by seed imbibition or foliar spray. In the third experiment, sixty-day-old rice plants grown in pots were challenge inoculated with Rhizoctonia solani strain TNAU-01. Methylobacterium inoculation promoted seed germination and plant growth. Increased plant height, number of tillers, plant biomass, and grain yield were observed. The average yield increases for seed imbibition and phyllosphere spray were, respectively, 22.1% and 24.3% greater than control. The bacteria also significantly reduced the sheath blight incidence when applied as either bacterial culture through seed imbibition and or phyllosphere spray. The percent disease reduction recorded for seed imbibition alone and for combined applications of seed imbibition and phyllosphere spray were 17.8% and 23.5%. Rice plants sprayed with PPFM-Os-07 strain showed increased presence of PR-proteins and phenolic contents on day 1 after application. Maximum phenylalanine ammonia lyase (PAL) and peroxidase activity on day 4 and β-1,3-glucanase and chitinase activity on day 5 were recorded. The results suggest that Methylobacterium inoculation may alter rice susceptibility to R. solani. This work emphasizes the importance of evaluating induced systemic resistance while studying plant-associated growth promoting bacteria.read more
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Metaproteogenomic analysis of microbial communities in the phyllosphere and rhizosphere of rice
Claudia Knief,Nathanaël Delmotte,Samuel Chaffron,Manuel Stark,Gerd Innerebner,Reiner Wassmann,Reiner Wassmann,Christian von Mering,Julia A. Vorholt +8 more
TL;DR: In this article, the phyllosphere and rhizosphere microbiota of rice cultivars were characterized using a metaproteogenomic approach to get insight into the physiology of the bacteria and archaea that live in association with rice.
Journal ArticleDOI
Regulation of ethylene levels in canola (Brassica campestris by 1 -aminocyclopropane -1 -carboxylate deaminase-containing Methylobacterium fujisawaense
TL;DR: The results collectively suggest that Methylobacterium commonly found in soils, as well as on the surfaces of leaves, seeds, and in the rhizosphere of a wide variety of plants could be better exploited to promote plant growth.
Journal ArticleDOI
Diversity of endophytic bacterial communities in poplar grown under field conditions.
TL;DR: The results showed that the genetic background of the hybrid poplar clones corresponded well with the endophytic community structure, and the community structure displayed clear differences in terms of the presence and relative proportions of bacterial taxa between the fourpoplar clones studied.
Journal ArticleDOI
Diversity of endophytic bacteria from Eucalyptus species seeds and colonization of seedlings by Pantoea agglomerans
Anderson J. Ferreira,Maria Carolina Quecine,Paulo Teixeira Lacava,Shinitiro Oda,João Lúcio de Azevedo,Welington Luiz Araújo,Welington Luiz Araújo +6 more
TL;DR: This is the first report of an endophytic bacterial community residing in Eucalyptus seeds and the transmission of these bacteria from seeds to seedlings, ensuring the support of the bacterial community in the host plant.
Journal ArticleDOI
Rice root-associated bacteria: insights into community structures across 10 cultivars
Pablo R. Hardoim,Pablo R. Hardoim,Fernando Dini Andreote,Barbara Reinhold-Hurek,Angela Sessitsch,Leonard S. van Overbeek,Jan Dirk van Elsas +6 more
TL;DR: In this paper, the effects of plant genotype, soil type and nutrient use efficiency on the composition of different bacterial communities associated with rice roots were investigated using PCR, followed by denaturing gradient gel electrophoresis (PCR-DGGE).
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