Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase T of Saccharomyces cerevisiae.
Rotraud Wieser,Gerhard Adam,Andreas Wagner,Christoph Schüller,G. Marchler,Helmut Ruis,Z Krawiec,T. Bilinski +7 more
Reads0
Chats0
TLDR
The results obtained show that expression of the Saccharomyces cerevisiae CTT1 gene is low at 23 degrees C and is induced rapidly at 37 degrees C, and Catalase T appears to have a function in protecting yeast cells against oxidative damage under stress conditions.About:
This article is published in Journal of Biological Chemistry.The article was published on 1991-07-05 and is currently open access. It has received 155 citations till now. The article focuses on the topics: Heat shock factor & Heat shock protein.read more
Citations
More filters
Journal ArticleDOI
Osmotic Stress Signaling and Osmoadaptation in Yeasts
TL;DR: An integrated understanding of osmoadaptation requires not only knowledge of the function of many uncharacterized genes but also further insight into the time line of events, their interdependence, their dynamics, and their spatial organization as well as the importance of subtle effects.
Journal ArticleDOI
The saccharomyces cerevisiae zinc finger proteins msn2p and msn4p are required for transcriptional induction through the stress-response element (stre )
María Teresa Martínez-Pastor,G Marchler,Christoph Schüller,Aron Marchler-Bauer,H Ruis,F Estruch +5 more
TL;DR: The results suggest that MSN2 and MSN4 encode a DNA‐binding component of the stress responsive system and it is likely that they act as positive transcription factors.
Journal ArticleDOI
MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae
TL;DR: The current knowledge of MAPK pathways in yeast is presented and some directions for future research in this area are presented, including how the upstream proteins actually activate the cascade remains unclear.
Journal ArticleDOI
Oxidative stress responses of the yeast Saccharomyces cerevisiae.
TL;DR: This review concentrates on the oxidant defence systems of the budding yeast Saccharomyces cerevisiae, which appears to have a number of inducible adaptive stress responses to oxidants, such as H2 O2 , superoxide anion and lipid peroxidation products.
Journal ArticleDOI
Trehalose accumulation during cellular stress protects cells and cellular proteins from damage by oxygen radicals.
TL;DR: Trehalose accumulation in stressed cells plays a major role in protecting cellular constituents from oxidative damage and decreased the initial appearance of damaged proteins by acting as a free radical scavenger.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Journal ArticleDOI
Transformation of intact yeast cells treated with alkali cations.
TL;DR: The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.