Journal ArticleDOI
Highly sensitive detection of proteins and bacteria in aqueous solution using surface-enhanced Raman scattering and optical fibers.
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TLDR
The detection of the proteins lysozyme and cytochrome c as well as the live bacterial cells of Shewanella oneidensis MR-1 in aqueous solutions with sensitivities order(s) of magnitude higher than those previously reported are reported.Abstract:
We report the detection of the proteins lysozyme and cytochrome c as well as the live bacterial cells of Shewanella oneidensis MR-1 in aqueous solutions with sensitivities order(s) of magnitude higher than those previously reported. Two highly sensitive surface-enhanced Raman scattering (SERS)-based biosensors using optical fibers have been employed for such label-free macromolecule detections. The first sensor is based on a tip-coated multimode fiber (TCMMF) with a double-substrate “sandwich” structure, and a detection limit of 0.2 μg/mL is achieved in protein detections. The second sensor is based on a liquid core photonic crystal fiber (LCPCF) with a better confinement of light inside the fiber core, and a detection limit of 106 cells/mL is achieved for the bacteria detection. Both SERS biosensors show great potential for highly sensitive and molecule-specific detection and identification of biomolecules.read more
Citations
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Journal ArticleDOI
Biosensors for whole-cell bacterial detection.
TL;DR: Recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing is discussed, with a particular focus on electrochemical biosensing systems.
Journal ArticleDOI
SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications
TL;DR: Focusing on several basic elements in SERS immunoassays, typical structures of SERS nanoprobes, productive optical spectral encoding strategies, and popular immunoASSay platforms are highlighted, followed by their representative biological applications in the last 5 years.
Journal ArticleDOI
Shell Thickness-Dependent Raman Enhancement for Rapid Identification and Detection of Pesticide Residues at Fruit Peels
Bianhua Liu,Guangmei Han,Guangmei Han,Zhongping Zhang,Renyong Liu,Changlong Jiang,Suhua Wang,Ming-Yong Han,Ming-Yong Han +8 more
TL;DR: The shell thickness-dependent Raman enhancement of silver-coated gold nanoparticles (Au@Ag NPs) for the identification and detection of pesticide residues at various fruit peels is reported.
Journal ArticleDOI
Recent progress in surface enhanced Raman spectroscopy for the detection of environmental pollutants
TL;DR: The surface enhanced Raman spectroscopy (SERS) has emerged as one of the most promising analytical tools in recent years as mentioned in this paper. But its performance has not yet reached the state-of-the-art.
Journal ArticleDOI
Isolation and identification of bacteria by means of Raman spectroscopy.
Susanne Pahlow,Susann Meisel,Dana Cialla-May,Karina Weber,Petra Rösch,Jürgen Popp,Jürgen Popp +6 more
TL;DR: The main emphasis of this work is on important aspects of applying Raman spectroscopy for the detection of bacteria like sample preparation and the identification process.
References
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Journal ArticleDOI
Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering
Shuming Nie,Steven R. Emory +1 more
TL;DR: In this article, surface-enhanced Raman scattering was used to detect single molecules and single nanoparticles at room temperature with the use of surface enhanced Raman, and the intrinsic Raman enhancement factors were on the order of 10 14 to 10 15, much larger than the ensemble-averaged values derived from conventional measurements.
Journal ArticleDOI
Single Molecule Detection Using Surface-Enhanced Raman Scattering (SERS)
Katrin Kneipp,Katrin Kneipp,Yang Wang,Yang Wang,Harald Kneipp,Harald Kneipp,Lev T. Perelman,Lev T. Perelman,Irving Itzkan,Irving Itzkan,Ramachandra R. Dasari,Ramachandra R. Dasari,Michael S. Feld,Michael S. Feld +13 more
TL;DR: In this article, the first observation of single molecule Raman scattering was made using a single crystal violet molecule in aqueous colloidal silver solution using one second collection time and about $2.
Journal ArticleDOI
Surface-enhanced spectroscopy
TL;DR: The surface-enhanced Raman scattering (SERS) effect was first discovered by Fleischmann, Van Duyne, Creighton, and Creighton as discussed by the authors, who showed that molecules adsorbed on specially prepared silver surfaces produce a Raman spectrum that is at times a millionfold more intense than expected.
Journal ArticleDOI
Adsorption and surface-enhanced Raman of dyes on silver and gold sols
P. C. Lee,Dan Meisel +1 more
TL;DR: Several negatively charged dyes were investigated for their possible adsorption on the surface of colloidal particles and those dyes that were found to adsorb on the particles were then checked for surface enhancement of Raman scattering.
Journal ArticleDOI
Surface-enhanced Raman scattering
TL;DR: In this paper, the basic experimental facts and the essential features of the mechanisms which have been proposed to account for the observations have been reviewed, including surface-enhanced Raman scattering (SERS) from single particles and single molecules.
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