Journal ArticleDOI
HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils
TLDR
In this paper, simple and fast HPLC methods in one-dimensional and two-dimensional dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils.Abstract:
The concentration of furocoumarins, known to induce mutagenesis, carcinogenesis and photodermatitis under UV radiation, is limited by the EU cosmetic regulation in consumer products such as cosmetics, sun protection and bronzing formulations. The bergapten concentration is also limited by the International Fragrance Association. Analytical methods have been proposed for their quantification using high-performance liquid chromatography (HPLC) coupled with UV detection or mass spectrometry with limits of detection (LOD) and quantification (LOQ) in accordance with the regulation. These methods are time and solvent consuming for routine quality control analyses. In this context, simple and fast HPLC methods in one- (1-D) and two- (2-D) dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils: psoralen, bergapten, xanthotoxin, isopimpinellin, oxypeucedanin, oxypeucedanin hydrate, byakangelicol, byakangelicin, heraclenin, 8-geranyloxypsoralen, bergamottin, imperatorin, isoimperatorin, phellopterin, epoxybergamottin along with five other furocoumarins known to be present in Angelica, bergamot or Heracleum species: angelicin, bergaptol, isobergapten, psora-4 and 5-(4-phenoxybutoxy)psoralen. The 1-D method was validated for the quantification of bergapten using a Camag TLC Scanner as a detector. The LOD was determined at 2 mg/kg. The method was linear from 2 to 100 mg/kg and the method range was defined using accuracy profiles at with an 80% tolerance interval, with a 30% error, to be 5–100 mg/kg in acetonitrile and 13–100 mg/kg in matrix.read more
Citations
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Journal ArticleDOI
Aromatic Profiles of Essential Oils from Five Commonly Used Thai Basils
TL;DR: The overall benefit from this research was the elucidation of aromatic qualities from Thai common Ocimum species in order to assess their potential as the raw materials for new food products.
Journal ArticleDOI
Coumarins, Psoralens and Polymethoxyflavones in Cold-pressed Citrus Essential Oils: a Review
TL;DR: In this article, cold-pressed Citrus essential oils namely lemon, bergamot, lime, grapefruit, mandarin, sweet and bitter orange are obtained from the peel of Citrus fruits.
Posted ContentDOI
Aromatic Profiles of Essential Oil from 5 Commonly Used Thai Basils
TL;DR: In this paper, the volatile compositions of different basil types available in Thai markets and descriptively determine their aromatic qualities were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) to qualitatively and quantitatively describe the chemical compositions.
References
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Journal ArticleDOI
Molecular and genetic basis of furocoumarin reactions.
Journal ArticleDOI
Furocoumarins in medicinal chemistry. Synthesis, natural occurrence and biological activity.
TL;DR: The scope of this review encompasses the importance of furocoumarins and the most important developments in this field that have been made in recent years, with particular emphasis placed on the aspects related to medicinal chemistry.
Journal ArticleDOI
Coumarins and antiplatelet aggregation constituents from Formosan Peucedanum japonicum.
Ih-Sheng Chen,Chin-Teng Chang,Wine-Show Sheen,Che-Ming Teng,Ian-Lih Tsai,Chang-Yih Duh,Feng-Nien Ko +6 more
TL;DR: Seven compounds, eugenin, (-)-selinidin, (+)-pteryxin, imperatorin, bergapten, cnidilin and (+)-visamminol, show strong antiplatelet aggregation activity in vitro.
Journal ArticleDOI
Quantification of total furocoumarins in citrus oils by HPLC coupled with UV, fluorescence, and mass detection.
Eric Frerot,Erik Decorzant +1 more
TL;DR: A reversed-phase HPLC method was developed to separate the 15 main furocoumarins present in citrus oils, and Quantification by UV, fluorescence, or mass detectors was compared in terms of linearity and limit of detection.
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