Identification, characterization and phylogenetic analysis of antifungal Trichoderma from tomato rhizosphere.
TLDR
There was a positive correlation between the antagonistic capacity of Trichoderma isolates towards fungal pathogens and their lytic enzyme production, and ERG-1 gene showed heterogeneity among some antagonistic isolates and indicated the possibility of occurrence of squalene epoxidase driven triterpene biosynthesis as an alternative biocontrol mechanism in Trichodma species.Abstract:
The use of Trichoderma isolates with efficient antagonistic activity represents a potentially effective and alternative disease management strategy to replace health hazardous chemical control. In this context, twenty isolates were obtained from tomato rhizosphere and evaluated by their antagonistic activity against four fungal pathogens (Fusarium oxysporum f. sp. lycopersici, Alternaria alternata, Colletotrichum gloeosporoides and Rhizoctonia solani). The production of extracellular cell wall degrading enzymes of tested isolates was also measured. All the isolates significantly reduced the mycelial growth of tested pathogens but the amount of growth reduction varied significantly as well. There was a positive correlation between the antagonistic capacity of Trichoderma isolates towards fungal pathogens and their lytic enzyme production. The Trichoderma isolates were initially sorted according to morphology and based on the translation elongation factor 1-α gene sequence similarity, the isolates were designated as Trichoderma harzianum, T. koningii, T. asperellum, T. virens and T. viride. PCA analysis explained 31.53, 61.95, 62.22 and 60.25% genetic variation among Trichoderma isolates based on RAPD, REP-, ERIC- and BOX element analysis, respectively. ERG-1 gene, encoding a squalene epoxidase has been used for the first time for diversity analysis of antagonistic Trichoderma from tomato rhizosphere. Phylogenetic analysis of ERG-1 gene sequences revealed close relatedness of ERG-1sequences with earlier reported sequences of Hypocrea lixii, T. arundinaceum and T. reesei. However, ERG-1 gene also showed heterogeneity among some antagonistic isolates and indicated the possibility of occurrence of squalene epoxidase driven triterpene biosynthesis as an alternative biocontrol mechanism in Trichoderma species.read more
Citations
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Antagonistic assessment of Trichoderma spp. by producing volatile and non-volatile compounds against different fungal pathogens
TL;DR: The results of present study confirmed that T. harzianum can be used as a promising biological control agent against Alternaria and Fusarium spp.
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Trichoderma for climate resilient agriculture.
TL;DR: The present mini-review is an effort to elucidate the molecular basis of plant growth promotion and defence activation by Trichoderma spp.
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Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth
TL;DR: Among non-pathogenic microorganisms, Trichoderma seems to be the best candidate for use in green technologies due to its wide biofertilization and biostimulatory potential.
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Biocontrol Potential of Salt-Tolerant Trichoderma and Hypocrea Isolates for the Management of Tomato Root Rot Under Saline Environment
Prem Lal Kashyap,Manoj Kumar Solanki,Manoj Kumar Solanki,Prity Kushwaha,Sudheer Kumar,Alok Kumar Srivastava +5 more
TL;DR: Application of salt-tolerant Trichoderma and Hypocrea isolates emerged as a simple, safe, and cheap method for the biological management of tomato root rot under saline condition.
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Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA.
TL;DR: In this paper, the potential application of mtDNA for pathogen diagnostics is discussed along with the potential use of mt DNA, including their strengths and weaknesses, and a brief description of qPCR and DNA barcoding as two major strategies enabling the diagnostics of plant pathogenic fungi and oomycetes.
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Biocontrol mechanisms of Trichoderma strains
TL;DR: The genus Trichoderma comprises a great number of fungal strains that act as biological control agents, the antagonistic properties of which are based on the activation of multiple mechanisms, such as plant growth factors, hydrolytic enzymes, siderophores, antibiotics, and carbon and nitrogen permeases.
Journal ArticleDOI
Trichoderma-based Products and their Widespread Use in Agriculture
Sheridan L. Woo,Michelina Ruocco,Francesco Vinale,Marco Nigro,Roberta Marra,Nadia Lombardi,Alberto Pascale,Stefania Lanzuise,Gelsomina Manganiello,Matteo Lorito +9 more
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Trichoderma: Systematics, the Sexual State, and Ecology
TL;DR: A chronology is presented that charts the development of a genus and species concept in Trichoderma, and a suggestion is made to modify the International Code of Botanical Nomenclature to enable adoption of a single generic name for Trichodma/Hypocrea.