Journal ArticleDOI
Identification of RAPD markers, in situ DNA content and structural chromosomal diversity in some legumes of the mangrove flora of Orissa
TLDR
By employing RAPD markers, karyotypes and 4C DNA content analysis, the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangrove that would shed light on the evolution ofMangroves from terrestrial species.Abstract:
Randomly amplified polymorphic DNA (RAPD) markers, karyotypes and 4C DNA content were analyzed in five legume mangroves belonging to the sub-family Papilinoideae (Dalbergia spinosa, Derris heterophylla and D. indica) and Caesalpinioideae (Caesalpinia crista, Cynometra ramiflora) of the family Fabaceae to establish the genetic variability and phylogenetic affinities. Somatic chromosome numbers were reported for the first time in D. spinosa (2n = 20), C. ramiflora (2n = 26) and D. heterophylla (2n = 24) with reconfirmation of the somatic chromosome number in D. indica (2n = 22) and C. crista (2n = 24). Significant intergeneric and interspecific variation of 4C DNA content was observed and that varied from 8.970 pg in C. ramiflora to 28.730 pg in D. indica. From the RAPD analysis, the dendogram showed clustering of Caesalpinia crista and Cynomitra ramiflora into one group (81.80). In the second groups Derris indica and Derris heterophylla were more similar (83.10) than Dalbergia spinosa (85.80). Species-specific DNA markers (900 bp) obtained in D. spinosa from OPN15; 700 and 2000 bp in C. ramiflora from OPN4 and 400 and 800 bp in D. heterophylla and 500 bp DNA fragment in C. crista obtained from OPN-11 were found characteristic RAPD markers of these species. C. crista found more closer affinity to C. ramiflora of the sub-family Caesalpinioideae [genetic distance (1-F) = 0.847]. Derris indica showed closer genetic relation with D. heterophylla [genetic distance (1-F) = 0.856] than D. spinosa [genetic distance (1-F) = 0.876] where Derris and Dalbergia belongs to the sub-family Papilionoideae. By employing these markers the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangroves that would shed light on the evolution of mangroves from terrestrial species.read more
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Molecular ecology and biogeography of mangrove trees towards conceptual insights on gene flow and barriers: A review
TL;DR: Recommendations on the molecular markers and on the sampling design of individuals and populations are given within a conceptual context of evolutionary significant units to increase the number of reliable comparisons of genetic variables across species or regions.
Journal ArticleDOI
Development and characterization of genomic and expressed SSRs for levant cotton (Gossypium herbaceum L.).
Satya Narayan Jena,Anukool Srivastava,Krishan Mohan Rai,Alok Ranjan,Sunil Kumar Singh,Tarannum Nisar,Meenal Srivastava,Sumit K. Bag,Shrikant Mantri,Mehar Hasan Asif,Hemant Kumar Yadav,Rakesh Tuli,Samir V. Sawant +12 more
TL;DR: This is the first report on the successful use of repeat enriched genomic library and expressed sequence database for microsatellite markers development in G. herbaceum.
Journal ArticleDOI
Large-scale resource development in Gossypium hirsutum L. by 454 sequencing of genic-enriched libraries from six diverse genotypes
Krishan Mohan Rai,Sunil Kumar Singh,Archana Bhardwaj,Verandra Kumar,Deepika Lakhwani,Apeksha Srivastava,Satya Narayan Jena,Hemant Kumar Yadav,Sumit K. Bag,Samir V. Sawant +9 more
TL;DR: This study contributes considerable amount of genomic resources and suggests a potential role of genic-enriched sequencing in genomic resource development for orphan crop plants.
Journal ArticleDOI
Analysis of genetic diversity, population structure and linkage disequilibrium in elite cotton (Gossypium L.) germplasm in India
Satya Narayan Jena,Anukool Srivastava,Uma Maheswar Singh,Sribash Roy,Nandita Banerjee,Krishan Mohan Rai,Sunil Kumar Singh,Verandra Kumar,Lal Babu Chaudhary,Joy Roy,Rakesh Tuli,Samir V. Sawant +11 more
TL;DR: The genetic diversity in tetraploid cotton species was found to be less than that in the diploid species, and a strong agreement was noticed between the clusters constructed on the basis of morphological and genotyping data.
Journal Article
Evaluation of genetic diversity in a natural rosewood population (Dalbergia nigra Vell. Allemão ex Benth.) using RAPD markers.
TL;DR: The degree of polymorphism was high, near that of other populations of similar species; however, it was considered low for the conservation of this species.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, version 2.1: Owner manual
F.J. Rohlf,F Rohlf +1 more
Journal ArticleDOI
Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics.
TL;DR: It is concluded that the rDNA sl variants and/or associated loci are under selection in CCII, which demonstrates that Rrn1 and Rrn2 are useful as new genetic markers.
Book
The botany of mangroves
TL;DR: The aim of this work is to contribute to the human awareness of the natural world and to contribute towards the humanizing of nature.