Journal ArticleDOI
Immunologic localization of the human seminal plasma protease inhibitor in human spermatozoa.
C. Hirschhäuser,S. Baudner +1 more
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This article is published in Fertility and Sterility.The article was published on 1972-06-01. It has received 11 citations till now. The article focuses on the topics: Protease inhibitor (biology) & Immunodiffusion.read more
Citations
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Journal ArticleDOI
Evidence for the location of the receptor-binding site of human erythropoietin at the carboxyl-terminal domain.
TL;DR: Five different peptides homologous to relatively hydrophilic regions of human erythropoietin (huEpo) have been synthesized to identify biologically active domains of the hormone, and only peptide-P2-induced antisera inhibited the biologic activity of rhuEpo in a cell proliferation assay, indicating that the carboxyl-terminal region of the molecule is essentially involved in theBiologic function of rhUEpo.
Journal ArticleDOI
Interaction of human sperm acrosomal proteinase with human seminal plasma proteinase inhibitors.
TL;DR: The results provide further evidence for the possible r\l=o^\le of the Fraction II inhibitor in the reproductive process.
Journal ArticleDOI
Interaction of immunoglobulin fragments with the mammalian sperm acrosome.
G. J. Allen,F. J. Bourne +1 more
TL;DR: It was shown that IgG interacted through the Fc fragment, the Fab fragment causing only weak staining of homologous spermatozoa, and this could be predominantly attributed to IgC, although there was strong staining due to IgM.
Journal ArticleDOI
Localization of seminal plasma proteinase inhibitors in human spermatozoa as revealed by the indirect immunofluorescence technique.
TL;DR: Present results suggest that they are attached at the plasma membrane in the case of human spermatozoa, which is relatively easily detached together with the membrane so that prefixation is necessary to achieve proper localization.
Journal ArticleDOI
The distribution of some hydrolytic enzymes in the semen of the domestic fowl, gallus domesticus
W. M. McINDOE,P. E. Lake +1 more
TL;DR: The distribution of some hydrolytic enzymes in fowl semen was studied and between-strain differences in the activity of enzymes, particularly acid phosphatase and trypsin-like enzyme were revealed.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Identification and subcellular localization of the enzymes effecting penetration of the zona pellucida by rabbit spermatozoa.
Richard Stambaugh,Jean Buckley +1 more
TL;DR: A sucrose density gradient centrifugation procedure for separating the subcellular components of washed and sonicated rabbit epididymal spermatozoa has been developed and applied to the sub cellular localization of several sperm enzymes, including the acrosomal enzymes involved in penetration of the zona pellucida by the spermatozoon.
Journal ArticleDOI
Inhibition of in Vitro Fertilization of Rabbit Ova by Trypsin Inhibitors
TL;DR: It is indicated that the acrosomal enzyme is the essential enzyme for penetration of the zona pellicida of the rabbit ovum and inhibition of in vitro fertilization.
Journal ArticleDOI
Relationship of a trypsin-like enzyme in rabbit spermatozoa to capacitation.
TL;DR: The existence of a trypsin-like enzyme (TLE) in acrosomes ofepididymal spermatozoa was confirmed and was further demonstrated to be present in acosomes of ejaculated and capacitated spermatozosa.
Journal ArticleDOI
Proteolytic reaction of mammalian spermatozoa on gelatin membranes.
TL;DR: The acrosomes of spermatozoa of several mammalian species show proteolytic activity when applied to fixed gelatin membranes, and the technique permits continuous observation of the enzymatic reaction of an individual spermatozoon.
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