Journal ArticleDOI
Improvement of somatic embryogenesis and plant recovery in cassava.
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TLDR
Methods for improving the efficiency of plant recovery from somatic embryos of cassava (Manihot esculenta Crantz) were investigated by optimizing the maturation regime and incorporating a desiccation stage prior to inducing germination.Abstract:
Methods for improving the efficiency of plant recovery from somatic embryos of cassava (Manihot esculenta Crantz) were investigated by optimizing the maturation regime and incorporating a desiccation stage prior to inducing germination. Somatic embryos were induced from young leaf lobes of in vitro grown shoots of cassava on Murashige and Skoog medium with 2,4-dichlorophenoxy acetic acid. After 15 to 20 days of culture on induction medium, the somatic embryos were transferred to a hormone free medium supplemented with activated charcoal. In another 18 days mature somatic embryos became distinctly bipolar and easily separable as individual units and were cultured on half MS medium for further development. Subsequent desiccation of bipolar somatic embryos resulted in 92% germination and 83% complete plant regeneration. The plants were characterized by synchronized development of shoot and root axes. Of the non-desiccated somatic embryos, only 10% germinated and 2% regenerated plants. Starting from leaf lobes, transplantable plantlets were derived from primary somatic embryos within 70 to 80 days.read more
Citations
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Journal ArticleDOI
The role of activated charcoal in plant tissue culture.
TL;DR: The effect of AC on growth regulator uptake is still unclear but some workers believe that AC may gradually release certain adsorbed products, such as nutrients and growth regulators which become available to plants.
Journal ArticleDOI
Secondary somatic embryogenesis and applications in plant breeding.
TL;DR: In most species somatic embryos can be converted into shoots, although the frequencies are mostly low, and somatics induced by growth regulator free or CYT supplemented media meet more difficulties in shoot development than embryos induced by AUX supplemented media.
Journal ArticleDOI
Development of friable embryogenic callus and embryogenic suspension culture systems in cassava (Manihot esculenta Crantz)
N. J. Taylor,Michelle Edwards,Rebecca J. Kiernan,Christopher D.M. Davey,D. Blakesley,Graham G. Henshaw +5 more
TL;DR: The availability of embryogenic suspension cultures is considered to have important implications for the application of genetic transformation and other biotechnologies in the agronomic improvement of cassava.
Journal ArticleDOI
Cassava genetic transformation and its application in breeding.
TL;DR: Major progress has been made in terms of an increased resistance to pests and diseases, biofortification, and improved starch quality, building on the fundamental knowledge and technologies related to planting, nutrition, and the processing of this important food crop that has often been neglected.
Book ChapterDOI
Somatic Embryogenesis in Herbaceous Dicots
TL;DR: The seed is the primary organ for the perpetuation of germplasm and propagation in all flowering plants and contains the embryo which develops from the zygote following the fertilization of the egg cell by one of the male gametes.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Gene expression in response to abscisic acid and osmotic stress.
Karen Skriver,John Mundy +1 more
TL;DR: Several ABA-responsive genes have been isolated and a major goal of the research discussed below is to understand the role these genes play in osmotic stress and desiccation tolerance.
Journal ArticleDOI
The Effect of Activated Charcoal on Tissue Cultures: Adsorption of Metabolites Inhibiting Morphogenesis
TL;DR: It was shown by mass spectrometry that the media without charcoal contained high amounts of phenylacetic acid and p-OH-benzoic acid (Daucus, Allium, and Haplopappus), whereas the media with activated charcoal did not.
Journal ArticleDOI
Enhanced Maturation and Desiccation Tolerance of White Spruce [Picea glauca (Moench) Voss] Somatic Embryos: Effects of a Non-plasmolysing Water Stress and Abscisic Acid
TL;DR: A non-plasmolysing moisture stress effected by polyethylene glycol (PEG) was beneficial when applied to maturing white spruce (Picea glauca) somatic embryos for the following reasons.
Journal ArticleDOI
Non-zygotic embryos of Brassica napus L. contain embryo-specific storage proteins.
TL;DR: Although storage proteins are present in both zygotic and non-zygotic embryos, the timing and extent of accumulation differ, and storage protein levels in microsporic and hypocotyl embryos were low relative to those in zygotics embryos.
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