Journal ArticleDOI
Non-zygotic embryos of Brassica napus L. contain embryo-specific storage proteins.
Reads0
Chats0
TLDR
Although storage proteins are present in both zygotic and non-zygotic embryos, the timing and extent of accumulation differ, and storage protein levels in microsporic and hypocotyl embryos were low relative to those in zygotics embryos.Abstract:
The storage-protein content of non-zygotic and zygotic embryos of B. napus was compared, using antibodies to guantitate 12S storage protein in extracts by rocket immunoelectrophoresis. Non-zygotic embryos were induced from microspores in anther culture and on the hypocotyls of zygotic embryos in culture. All embryo-like structures were found to contain 12S storage protein, whereas preculture anthers, anthers from which embryos had been removed, and regenerated shoots did not have detectable 12S storage protein. In zygotic embryos, 12S storage protein was first detected at the cotyledon stage, but microsporic embryos contained storage protein at the globular and heart stages. Storage protein levels in microsporic and hypocotyl embryos were low relative to those in zygotic embryos. The largest microsporic embryo had a storage protein concentration of 13 μg mg-1 fresh weight, almost 10 times lower than a mature zygotic embryo. Thus, although storage proteins are present in both zygotic and non-zygotic embryos, the timing and extent of accumulation differ.read more
Citations
More filters
Journal ArticleDOI
Regulation of gene expression during plant embryogenesis.
Journal ArticleDOI
Hormones and the physiological architecture of life history evolution.
Caleb E. Finch,Michael R. Rose +1 more
TL;DR: It is argued that the MHC and other complex loci may be considered as life history gene complexes, with pleiotropic influences throughout the lifespan, and the population genetics that is involved provides a theoretical basis for great flexibility in hormonal regulation during life history evolution.
Journal ArticleDOI
Regulatory mechanisms involved in the transition from seed development to germination.
TL;DR: In this paper, the transition from seed development to germination is discussed, and the regulatory mechanisms involved in the transition are discussed, as well as a discussion of the role of genetic information in the process.
Book ChapterDOI
Morphogenic Aspects of Somatic Embryogenesis
TL;DR: Credit for the first descriptions of somatic embryogenesis might more properly belong to Levine, who in 1947 reported the recovery of carrot “seedlings” from tissues exposed to low levels of α-naphthaleneacetic acid, via a process whose description sounds very much like somatic embryos.
Journal Article
Zygotic embryogenesis versus somatic embryogenesis
TL;DR: This review will summarize molecular and genetic analyses aimed at identifying the mechanisms underlying the sequence of events during plant zygotic embryogenesis, being studied in parallel with the histological and morphological analyses of somatic embryogenesis.
References
More filters
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Polypeptides of the tail fibres of bacteriophage T4
Jonathan King,Ulrich K. Laemmli +1 more
TL;DR: The products of four of the six genes involved in bacteriophage T4 tail fibre assembly by sodium dodecyl sulphate-acrylamide gel electrophoresis of tail fibre mutant lysates and particles purified from them are identified, allowing the formulation in greater detail of the early stages of the fibre assembly pathway.
Journal ArticleDOI
Development and storage-protein synthesis in Brassica napus L. embryos in vivo and in vitro
Martha L. Crouch,Ian M. Sussex +1 more
TL;DR: Results indicate that some factor in the seed environment is necessary for storage-protein synthesis to proceed, and that ABA is a possible candidate.
Journal ArticleDOI
In vitro production of plants from pollen in brassica campestris
TL;DR: Embryogenesis was induced in anthers of two cultivars of B. campestris L. cultured on a modified B5 medium of Gamborg and co-workers (1968) and analysis of mature plants derived from the embryoids rev...
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more