Journal ArticleDOI
Improvements in the isolation procedure for thyroid peroxidase: nature of the heme prosthetic group.
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TLDR
In this paper, an improved procedure for the solubilization and purification of hog thyroid peroxidase is described, based on the results of the spectral analysis of the protein bands associated with the most intensely staining protein bands.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-07-01. It has received 87 citations till now. The article focuses on the topics: Cytochrome c peroxidase & Horseradish peroxidase.read more
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Journal ArticleDOI
The molecular biology of thyroid peroxidase: cloning, expression and role as autoantigen in autoimmune thyroid disease.
TL;DR: Three of the four major thyroid-specific proteins have been cloned, namely thyroglobulin (TG), TPO (see below), and the TSH receptor (5–8), and molecular cloning of the thyroid iodide transporter may occur soon (9).
Journal ArticleDOI
Structural and functional aspects of thyroid peroxidase.
Jean Ruf,Pierre Carayon +1 more
TL;DR: The latest contributions in the field of TPO research are reviewed and a large reference list of original publications is provided to investigate the mechanisms whereby TPO contributes to hormone synthesis and constitutes an important autoantigen involved in autoimmune thyroid disease.
Journal ArticleDOI
Biosynthesis of thyroid hormone: Basic and clinical aspects☆
TL;DR: Thyroid hormone formation requires the coincident presence of peroxidase, H2O2, iodide, and acceptor protein at one anatomic locus in the cell, and clinical problems involving defective per oxidase function are among the most frequent hereditary defects of thyroid hormone formation.
Book ChapterDOI
Formation of thyroid hormones.
TL;DR: The metabolic pathway that allows the synthesis of thyroid hormones from inorganic iodide has been extensively documented at the physiological level, the investigations being greatly facilitated by the use of the iodine isotopes.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
Purification of horse-radish peroxidase and comparison of its properties with those of catalase and methaemoglobin.
David Keilin,E. F. Hartree +1 more
Journal ArticleDOI
The Isolation and Purification of Thyroid Peroxidase
Toichiro Hosoya,Martin Morrison +1 more
TL;DR: The spectral properties suggest that the enzyme is a typical hemoprotein peroxidase, while gel filtration experiments have shown it to have a molecular weight of 104,000, and the specific activity of the preparation was significantly higher than that of other reported preparations.