In vitro anticancer, anti-inflammatory, and antioxidant potentials of Ephedra aphylla
Wajdy Al-Awaida,Baker Jawabrah Al-Hourani,Muhanad Akash,Wamidh H. Talib,Sima Zein,Rabah Rashad Falah,Zaid Aburubaiha +6 more
TLDR
E. aphylla has a promising potential to be used as a drug source for breast cancer treatment based on its strong antiproliferative activity.Abstract:
Purpose: The goal of our study is to test whether a naturally occurring plant, Ephedra aphylla, will show antiproliferative ability against tested cell lines and to test its anti-inflammatory and antioxidative potentials. Materials and Methods: In our study, we used four solvents with different polarities – aqueous, chloroform, methanol, and n-hexane – to extract different compounds from the aerial parts of E. aphylla. Antioxidant activity of E. aphylla was determined by measuring nitric oxide (NO) and hydrogen peroxide (H2O2) scavenging activities. The anti-inflammatory activity was studied using the inhibition of albumin denaturation assay. Finally, the antiproliferative activity of breast cancer cell lines (T47D, MCF-7) and Vero cell line (African green monkey kidney) was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: Phytochemical screening for various extracts of E. aphylla showed the presence of medicinally important compounds including cardiac glycosides, alkaloids, triterpenes, tannins, and flavonoids. The scavenging activity for H2O2 of various solvent extracts was in the order of methanol > aqueous > chloroform > ethyl acetate > n-hexane. In addition, E. aphylla solvent extracts also exhibited a scavenging activity for NO in the order of methanol > ethyl acetate > aqueous > chloroform > n-hexane. All of the solvent extracts showed IC50 inhibition of albumin denaturation at a concentration between 209.5 ± 8.1 and 225 ± 11 μg/ml. Moreover, all extracts displayed strong antiproliferative potential against MFC7, T47D tested cell lines and very weak cytotoxic activity against Vero normal cell line. Conclusions: E. aphylla has a promising potential to be used as a drug source for breast cancer treatment based on its strong antiproliferative activity.read more
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Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line
Corina Danciu,Delia Muntean,Ersilia Alexa,Claudia Farcas,Camelia Oprean,István Zupkó,Andrea Bor,Daliana Minda,Maria Proks,Valentina Buda,Monica Hancianu,Oana Cioanca,Codruta Soica,Sofia Popescu,Cristina Dehelean +14 more
TL;DR: The results show that the hydroalcoholic extract of the aerial part of Ephedra alata Decne contains polyphenolic phytocompounds and elicits antioxidant activity, and presents antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 human breast cancer cell line.
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A Review on Worldwide Ephedra History and Story: From Fossils to Natural Products Mass Spectroscopy Characterization and Biopharmacotherapy Potential.
Khaoula Elhadef,Slim Smaoui,Mariam Fourati,Hajer Ben Hlima,Ahlem Chakchouk Mtibaa,Imen Sellem,Karim Ennouri,Lotfi Mellouli +7 more
TL;DR: An exhaustive review of the scientific literature on biomedicine and pharmacotherapy (anticancer, antiproliferative, anti-inflammatory, antidiabetic, antihyperlipidemic, antiarthritic, and anti-influenza activities; proapoptotic and cytotoxic potential; and so on) and antimicrobial and antioxidant activities were discussed.
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The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract.
Mustafa El-Zayat,Mostafa M. Eraqi,Hani Alrefai,Ayman Y. El-Khateeb,Marwan A. Ibrahim,Hashim M. Aljohani,Maher M. Aljohani,Moustafa M. Elshaer +7 more
TL;DR: In this paper, the authors synthesize selenium and zinc nanoparticles using the aqueous extract of Ephedra aphylla as a valuable medicinal plant, and the prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents.
Journal ArticleDOI
A New Highlight of Ephedra alata Decne Properties as Potential Adjuvant in Combination with Cisplatin to Induce Cell Death of 4T1 Breast Cancer Cells In Vitro and In Vivo.
Fairouz Sioud,Fairouz Sioud,Fairouz Sioud,Souheila Amor,Souheila Amor,Imene Ben Toumia,Aida Lahmar,Virginie Aires,Virginie Aires,Leila Chekir-Ghedira,Dominique Delmas,Dominique Delmas +11 more
TL;DR: Very interestingly, a synergic antiproliferative and pro-apoptotic action with cisplatin was observed, associated with a strong decrease of breast tumor growth in mice treated with an E.A.A./cisplatin combination and simultaneously with a decrease of hepato- and nephrotoxicities of cis platin.
Journal ArticleDOI
Chemical Composition, Antimicrobial, Hemolytic, and Antiproliferative Activity of Essential Oils from Ephedra intermedia Schrenk & Mey
TL;DR: EIO presented anti-proliferative effect on Hela, HCT116, and LnCap cells with mortality of 67 % to 81 %, and the safety of EIO as an additive for different aspects can be considered.