Journal ArticleDOI
In vitro propagation of Iphigenia indica, an alternative source of colchicine
TLDR
The present study involves in vitro propagation of Iphigenia indica (Kunth.) through multiplication of whole corms and corm buds to produce numerous shoots at variable rates in presence of α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP).Abstract:
The present study involves in vitro propagation of Iphigenia indica (Kunth.) through multiplication of whole corms and corm buds. The whole corms produced very small micro-corms, which developed plants individually whereas corm buds multiplied to produce numerous shoots at variable rates in presence of α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). The best response in corm and bud multiplication was obtained in Murashige and Skoog's basal medium (MS) supplemented with 2.69 μM NAA and 8.88 μM BAP. The shoots regenerated were further cultured on MS medium containing NAA and indole-3-butyric acid (IBA) for initiation of roots. MS medium with 5.38 μM NAA and 4.92 μM IBA induced highest percentage of roots (81%) within 2 weeks in culture.read more
Citations
More filters
Journal Article
Influence of cultivar, explant source and plant growth regulator on callus induction and plant regeneration of cannabis sativa l.
TL;DR: Significant improvement of hemp plant regeneration in vitro was achieved, and the highest number of plants was regenerated from callus tissue of petiole explants on MS medium containing DICAMBA.
Journal ArticleDOI
In vitro stable regeneration of onion and garlic from suspension culture and chromosomal instability in solid callus culture
TL;DR: Chromosome analysis of the regenerated plants revealed stable chromosome number (2n = 16) in both species and callus tissues maintained in solid induction medium for long period showed abnormality in chromosome behavior leading to the formation of both hypo- and hyper-diploids cells along with the diploid cells.
Journal Article
Biomolecular changes during in vitro organogenesis of Asteracantha longifolia (L.) Nees--a medicinal herb.
TL;DR: High frequency plant regeneration in A. longifolia was achieved from leaf explant implanted on MS basal medium supplemented with NAA + BA through intervening callus phase and protein and total soluble sugar contents were maximum during organogenesis and multiple shoot induction phase compared with non-organogenic callus and root induction phase.
Journal ArticleDOI
Clonal propagation of Zephyranthes grandiflora using bulbs as explants
TL;DR: The morphologically identical plants derived by in vitro propagation were genetically identical as shown by PCR based ISSR marker analysis of genomic DNA.
Journal ArticleDOI
In Vitro Clonal Propagation Through Bud Culture of Hemidesmus indicus (L) R Br: An Important Medicinal Herb
TL;DR: In vitro propagation of Hemidesmus indicus (L) R Br through bud multiplication and subsequent plant regeneration and Chromosome number stability with no detectable structural changes was observed in the regenerates.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal Article
Micropropagation of Gloriosa superba L. - an endangered species of Asia and Africa.
Journal ArticleDOI
Optimization of callus culture and shoot multiplication of Asparagus densiflorus
TL;DR: Calluses on medium 1 were soft and friable, whereas, compact, hard calluses originated on medium 2, and Kinetin was found to be less effective than BA in the initiation of shoots (1.8 shoots/callus).
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more