Journal ArticleDOI
In vitro propagation of Saussurea obvallata (DC.) Edgew.—an endangered ethnoreligious medicinal herb of Himalaya
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Application of this micropropagation protocol for Saussurea obvallata (DC.) Edgew has the potential to substantially reduce the pressure on natural populations.Abstract:
This is the first report of a micropropagation protocol for Saussurea obvallata (DC.) Edgew. (Asteraceae), a rare, threatened and near-endemic medicinal herb of the Indian Himalayan region. Multiple shoots were formed from epicotyle explants on Murashige and Skoog (MS) medium supplemented with 1.0 µM kinetin and 0.25 µM α-naphthaleneacetic acid. A maximum of five shoots were obtained from one explant in a 75-day culture period. The effect of subsequent subcultures on shoot formation was also studied. After 100% in vitro rooting was obtained in half-strength MS supplemented with 2.5 µM Indole-3-butyric acid, the plantlets were transferred to ex vitro conditions. Following a 15-day in vitro rooting period and 12 days of ex vitro acclimatization, 66.7% of the plantlets had established in the field. Application of this protocol has the potential to substantially reduce the pressure on natural populationsread more
Citations
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Journal ArticleDOI
Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators.
Uppeandra Dhar,Mitali Joshi +1 more
TL;DR: Application of this protocol has potential for mass multiplication of the target species in a limited time period and reflected the existence of high inter-explant variability in response to growth regulators.
Journal ArticleDOI
In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.
Bin Guo,Min Gao,Chun-Zhao Liu +2 more
TL;DR: The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant.
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TL;DR: This is a first report of regeneration of Jatropha curcas with additives especially with L-arginine, which is a medicinal as well as a promising plant for biofuel production.
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In vitro clonal propagation through mature nodes of Tinospora cordifolia (Willd.) Hook. F. & Thoms.: An important ayurvedic medicinal plant
TL;DR: Of the two basal media tested, WPM was found to be superior to MS medium for the induction of multiple shoots and N6-benzyladenine (BA) was more effective than KIN for axillary shoot proliferation and KIN was superior to BA in terms of shoot elongation.
Journal ArticleDOI
An efficient plant regeneration system for mucuna pruriens l. (dc.) using cotyledonary node explants
TL;DR: An efficient micropropagation system for Mucuna pruriens, an important medicinal plant in India, was developed using a range of cytokinins investigated for multiple shoot regeneration with cotyledonary node explants from 7-d-old aseptic seedlings.
References
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
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Plant cell culture : a practical approach
TL;DR: A comparison of protoplast technology, which enabled plant regeneration via embryonic suspension cultures and in vitro selection for disease/toxin resistance, and the results of studies with viruses, show the need for further research into this technology.
Journal ArticleDOI
In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.).
TL;DR: In vitro plants were successfully established in the field and were morphologically uniform and a simple method to extend the subculture interval was used and its relevance to germplasm conservation is discussed.
Journal ArticleDOI
Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb
TL;DR: In vitro clonal multiplication of safed musli (Chlorophytum borivilianum Sant. Fernand.), a rare Indian medicinal herb, has been achieved on Murashige and Skoog's MS medium supplemented with 22.2 μM benzyladenine using young shoot bases as explants.
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