Influence of partial deletion of the Y chromosome on mouse sperm phenotype.
J. Styrna,J. Klag,K. Moriwaki +2 more
Reads0
Chats0
TLDR
Electron microscope analysis showed that some of the round, early spermatids in the mutants had normally formed acrosomal caps but lacked the proacrosomal granule and had no, or only scarce, acrosome material.Abstract:
Two congenic strains of mice (control, B10.BR/SgSn; mutant, B10.BR-Ydel/Ms with partial deletion of the Y chromosome) were examined. In control males, 22.6% of spermatozoa had abnormal heads; in mutant males, there were 64.2%, the most common being heads with flat acrosomes. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of mature sperm proteins, followed by acrosin assay and acrosome silver staining, revealed a reduced concentration of acrosin in acrosomal caps in 35.8% of the spermatozoa in mutant males. Electron microscope analysis showed that some of the round, early spermatids in the mutants had normally formed acrosomal caps but lacked the proacrosomal granule and had no, or only scarce, acrosomal material. These observations indicate that formation of the acrosomal cap is controlled separately from the synthesis of the acrosomal material and suggest that some factors linked on the Y chromosome are involved in the control of acrosome development.read more
Citations
More filters
Journal ArticleDOI
Sequencing the Mouse Y Chromosome Reveals Convergent Gene Acquisition and Amplification on Both Sex Chromosomes
Y. Q. Shirleen Soh,Jessica Alföldi,Tatyana Pyntikova,Laura G. Brown,Laura G. Brown,Tina Graves,Patrick Minx,Robert S. Fulton,Colin Kremitzki,Natalia Koutseva,Jacob L. Mueller,Steve Rozen,Jennifer F. Hughes,Elaine Owens,James E. Womack,William J. Murphy,Qing Cao,Pieter J. de Jong,Wesley C. Warren,Richard K. Wilson,Helen Skaletsky,Helen Skaletsky,David C. Page,David C. Page +23 more
TL;DR: The complete mouse MSY sequence brings to light dramatic forces in sex chromosome evolution: lineage-specific convergent acquisition and amplification of X-Y gene families, possibly fueled by antagonism between acquired X-y homologs.
Journal ArticleDOI
Fertility in mice requires X-Y pairing and a Y-chromosomal "spermiogenesis" gene mapping to the long arm.
TL;DR: It is concluded that the Y-specific region of the mouse Y chromosome long arm includes information essential for the normal development of the sperm head.
Journal ArticleDOI
The multicopy gene Sly represses the sex chromosomes in the male mouse germline after meiosis.
Julie Cocquet,Peter J.I. Ellis,Yasuhiro Yamauchi,Shantha K. Mahadevaiah,Nabeel A. Affara,Monika A. Ward,Paul S. Burgoyne +6 more
TL;DR: Small-interfering RNAs have been used to disrupt the function of the more than 100 copies of the Sly gene on the mouse Y chromosome, leading to defective sex chromosome repression during spermatid differentiation and, as a consequence, sperm malformations and near-sterility.
Journal ArticleDOI
The Y chromosome as a regulatory element shaping immune cell transcriptomes and susceptibility to autoimmune disease
Laure K. Case,Emma H. Wall,Julie A. Dragon,Naresha Saligrama,Dimitry N. Krementsov,Mohamad Moussawi,James F. Zachary,Sally A. Huber,Elizabeth P. Blankenhorn,Cory Teuscher +9 more
TL;DR: Data are established that susceptibility to two diverse animal models of autoimmune disease, experimental allergic encephalomyelitis (EAE) and experimental myocarditis, correlates with the natural variation in copy number of Sly and Rbmy multicopy ChrY genes.
Journal ArticleDOI
The Biology and Evolution of Mammalian Y Chromosomes.
Jennifer F. Hughes,David C. Page +1 more
TL;DR: In this paper, a third biological theme emerges from recent insights into the Y chromosome's roles beyond the reproductive tract, a theme that promises to broaden the reach of Y-chromosome research by shedding light on fundamental sex differences in human health and disease.