Journal ArticleDOI
Influence of selenium on glutathione and some associated enzymes in rats with mammary tumor induced by 7,12-Dimethylbenz(a)anthracene
TLDR
Observations clearly demonstrate the influence of dietary selenium supplementation in correcting abnormal changes in glutathione turnover and some associated enzymes in tumor induced rats.Abstract:
A recent finding in epidemiological and laboratory studies suggests that the ratio of selenium to glutathione is lower in breast cancer subjects than its control counterparts. Selenium, an antioxidant and anticarcinogen, can modify the status of glutathione and some associated enzymes by blocking peroxidation of lipids in membranes of cancer subjects. Studies were conducted using female albino rats of Wistar strain bearing mammary tumor induced by 7,12-dimethylbenz(a) anthracene to assess the biological role of selenium on some antioxidant enzymes associated with the maintenance of glutathione status. For induction of mammary tumor, 25 mg DMBA in a 1 ml emulsion of sunflower oil and physiological saline was injected subcutaneously to each rat. One group in each of control and tumor bearing rats, were fed 5 mg sodium selenite/kg diet from the day of tumor induction for 24 weeks. Increase in the reduced glutathione concentration was preceded by significant increase in the oxidized glutathione as well as in the activities of γ-glutamylcysteine synthetase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, and glucose-6-phosphate dehydrogenase by selenium administration in rats bearing tumor. However, selenium administration to rats bearing tumor decreased the activity of γ-glutamyl transpeptidase. These observations clearly demonstrate the influence of dietary selenium supplementation in correcting abnormal changes in glutathione turnover and some associated enzymes in tumor induced rats.read more
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Heritability of Some Important Parameters of the Antioxidant Defense System Like Glucose-6-Phosphate Dehydrogenase, Catalase, Glutathione Peroxidase and Lipid Peroxidation in Red Blood Cells by Twin Study
TL;DR: Data on the important parameters of antioxi- dant defense system like glucose-6-phosphate dehydroge- nase, catalase, glutathione peroxidase and lipid peroxidation from 7 pairs of monozygotic and like-sexed male twin pairs were subjected to variance analysis to examine significance of those biochemical traits.
Journal ArticleDOI
In vivo and in vitro studies evaluating the chemopreventive effect of metformin on the aryl hydrocarbon receptor-mediated breast carcinogenesis
Ali Alhoshani,Moureq R. Alotaibi,Homood M. As Sobeai,Naif O. Al-Harbi,Khalid Alhazzani,Abdullah Al-Dhfyan,Fawaz E. Alanazi,Hesham M. Korashy +7 more
TL;DR: Evidence is reported that MET is an effective chemopreventive therapy for breast cancer by inhibiting the activation of CYP1A1/CYP1B1 pathway in vivo rat model.
Journal ArticleDOI
Effects of Formononetin on the Aryl Hydrocarbon Receptor and 7,12-Dimethylbenz[a]anthracene-induced Cytochrome P450 1A1 in MCF-7 Human Breast Carcinoma Cells
TL;DR: The results suggest that formononetin might be considered as a natural ligand to bind on AhR and consequently produces a potent protective effect against DMBA-induced genotoxicity.
Inhibition of the in Vitro Growth of Human Mammary Carcinoma Cell Line (MCF-7) by Selenium and Vitamin E
TL;DR: The effects of the natural form of vitamin E; alpha tocopherol and two forms of selenium; sodium selenite and selenomethionine on the in vitro growth of the human mammary cancer cell line; MCF-7 were investigated as mentioned in this paper.
RESEARCH COMMUNICATION Antitumoral Effects of Melissa officinalis on Breast Cancer in Vitro and in Vivo
Serpil Ünver Saraydin,Ersin Tuncer,Sule Karadayi,Kursat Karadayi,Deniz Sahin Inan,Zübeyde,Mustafa Duman,Mustafa Turan +7 more
TL;DR: In vivo studies indicated that MO extrcts have antitumoral potential against breast cancer, showing cytotoxicity against three cancer cell lines, inducing increase in Annexin-positive cells.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
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Journal ArticleDOI
Selenium: Biochemical Role as a Component of Glutathione Peroxidase
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Journal ArticleDOI
Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues
TL;DR: The use of the foregoing analytical method in the determination of total and oxidized glutathione contents of rat blood, kidney, and liver gave values in good agreement with those obtained by previous investigators.