Journal ArticleDOI
Influence of zinc on protein metabolism in various lung cell lines.
U.I. Walther,W. Forth +1 more
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TLDR
Autoradiographically detectable methionine in 2D-gels obtained after exposure of cells to high zinc concentrations for time intervals greater than 1hr showed a time-dependent decrease in all cell lines, and few additional spots that could be heat shock proteins in alveolar epithelial cell lines after zinc exposure were obtained.About:
This article is published in Toxicology in Vitro.The article was published on 1999-12-01. It has received 3 citations till now. The article focuses on the topics: Zinc toxicity & Zinc.read more
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Journal ArticleDOI
Toxicity pathway focused gene expression profiling of PEI-based polymers for pulmonary applications.
TL;DR: In view of the lung as a sensitive target organ this inflammatory stimulation might cause unwanted side effects related to respiratory and cardiovascular disorders, so further optimization of the PEI-based vector systems is still needed for pulmonary application.
Journal ArticleDOI
Effect of low level zinc pretreatment on zinc-mediated toxicity in different lung cell lines
U. I. Walther,Harald Mückter,B. Wallner,U. Bergen,S. Duggen,G. Unsinn,S. C. Walther,B. Fichtl +7 more
TL;DR: It is concluded that the altered zinc tolerance of pretreated cells is not attributable to the induction of metallothionein, and protective effects by zinc have been shown for a variety of toxicity studies dealing with cadmium, in vitro and in vivo.
Journal ArticleDOI
Lack of effects of hydrocortisone pretreatment on zinc-induced changes in protein assemble.
TL;DR: The virtual lack of major effects does not support the hypothesis that a gross interaction between glucocorticoids and zinc at the cellular protein synthesis level would be an important mechanism of influence in zinc-induced lung injury.
References
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Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
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Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
TL;DR: A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development.
Journal ArticleDOI
Protein folding in the cell.
TL;DR: Folding and assembly of polypeptides in vivo involves other proteins, many of which belong to families that have been highly conserved during evolution.
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THE HEME OXYGENASE SYSTEM:A Regulator of Second Messenger Gases
TL;DR: This review highlights the current information on molecular and biochemical properties of HO-1 and HO-2 and addresses the possible mechanisms for mutual regulatory interactions between the CO- and NO-generating systems.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.