Inhibition of citrinin-induced apoptotic biochemical signaling in human hepatoma G2 cells by resveratrol.
Chia Chi Chen,Wen-Hsiung Chan +1 more
Reads0
Chats0
TLDR
It is demonstrated that CTN stimulates ROS generation and JNK activation for mitochondria-dependent apoptotic signaling in Hep G2 cells, and these apoptotic biochemical events are blocked by pretreatment with resveratrol, which exerts antioxidant effects.Abstract:
The mycotoxin citrinin (CTN), a natural contaminant in foodstuffs and animal feeds, exerts cytotoxic and genotoxic effects on various mammalian cells. CTN causes cell injury, including apoptosis, but its precise regulatory mechanisms of action are currently unclear. Resveratrol, a member of the phytoalexin family found in grapes and other dietary plants, possesses antioxidant and anti-tumor properties. In the present study, we examined the effects of resveratrol on apoptotic biochemical events in Hep G2 cells induced by CTN. Resveratrol inhibited CTN-induced ROS generation, activation of JNK, loss of mitochondrial membrane potential (MMP), as well as activation of caspase-9, caspase-3 and PAK2. Moreover, resveratrol and the ROS scavengers, NAC and α-tocopherol, abolished CTN-stimulated intracellular oxidative stress and apoptosis. Active JNK was required for CTN-induced mitochondria-dependent apoptotic biochemical changes, including loss of MMP, and activation of caspases and PAK2. Activation of PAK2 was essential for apoptosis triggered by CTN. These results collectively demonstrate that CTN stimulates ROS generation and JNK activation for mitochondria-dependent apoptotic signaling in Hep G2 cells, and these apoptotic biochemical events are blocked by pretreatment with resveratrol, which exerts antioxidant effects.read more
Citations
More filters
Journal ArticleDOI
Resveratrol and liver: A systematic review.
TL;DR: These high quality preclinical studies propose the potential therapeutic implication of resveratrol in liver disorders especially those with hepatic steatosis by reducing hepatic fibrosis through modulating the insulin resistance and lipid profile in animals.
Journal ArticleDOI
Review: Biotechnology of mycotoxins detoxification using microorganisms and enzymes
Fadia Ben Taheur,Bochra Kouidhi,Yasir Mohammed A. Al Qurashi,Jalila Ben Salah-Abbès,Kamel Chaieb +4 more
TL;DR: Recent development in biological control of mycotoxins removal and detoxification will be discussed and bacteria, yeast, moulds and enzymes may be used as a novel strategy for mycotoxin removal.
Journal ArticleDOI
Hepatotoxic effect of ochratoxin A and citrinin, alone and in combination, and protective effect of vitamin E: In vitro study in HepG2 cell
Loganathan Gayathri,Rajakumar Dhivya,Dharumadurai Dhanasekaran,Vaiyapuri Subbarayan Periasamy,Ali A. Alshatwi,Mohammad Abdulkader Akbarsha,Mohammad Abdulkader Akbarsha +6 more
TL;DR: Findings indicate that OTA and CTN in combination affect hepatocytes at very low concentrations and, thereby, pose a potential threat to public and animal health.
Journal ArticleDOI
A comprehensive review on biological properties of citrinin.
José Williams Gomes de Oliveira Filho,Muhammad Torequl Islam,Eunus S. Ali,Shaikh Jamal Uddin,José Victor de Oliveira Santos,Marcus Vinícius Oliveira Barros de Alencar,Antonio Luiz Gomes Júnior,Márcia Fernanda Correia Jardim Paz,Maria dos Remédios Mendes de Brito,João Marcelo de Castro e Sousa,Subrata Shaw,Maria das Graças Freire de Medeiros,Sandra Maria Mendes de Moura Dantas,Hercília Maria Lins Rolim,Paulo Michel Pinheiro Ferreira,Mohammad Amjad Kamal,Magdalena D. Pieczynska,Niranjan Das,Vijai Kumar Gupta,Andrei Mocan,Teresinha de Jesus Aguiar dos Santos Andrade,Brahma N. Singh,Siddhartha Kumar Mishra,Atanas G. Atanasov,Atanas G. Atanasov,Ana Amélia de Carvalho Melo-Cavalcante +25 more
TL;DR: A systematic review presents the current state of CIT research with emphasis on its bioactivity profile and reports that it is reported to possess a broad spectrum of bioactivities, including antibacterial, antifungal, and potential anticancer and neuro-protective effects in vitro.
Journal ArticleDOI
Citrinin-Generated Reactive Oxygen Species Cause Cell Cycle Arrest Leading to Apoptosis via the Intrinsic Mitochondrial Pathway in Mouse Skin
TL;DR: Dermal exposure to CTN was undertaken to study oxidative stress, DNA damage, cell cycle arrest, and apoptosis in mouse skin, and pretreatment with bio-antioxidants are suggested to be useful in the prevention of CTN-induced dermal toxicity.
References
More filters
Journal ArticleDOI
Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis
TL;DR: The effects of dominant-interfering or constitutively activated forms of various components of the JNK-p38 and ERK signaling pathways demonstrated that activation of JNK and p38 and concurrent inhibition of ERK are critical for induction of apoptosis in these cells.
Book ChapterDOI
Role of free radicals and catalytic metal ions in human disease: an overview.
TL;DR: The chapter discusses the metabolism of transition metals, such as iron and copper, and the chelation therapy that is an approach to site-specific antioxidant protection.
Journal ArticleDOI
Cancer Chemopreventive Activity of Resveratrol, a Natural Product Derived from Grapes
Mei-Shiang Jang,Lining Cai,George Udeani,Karla Slowing,Cathy F. Thomas,Chris Beecher,Harry H. S. Fong,Norman R. Farnsworth,A. Douglas Kinghorn,Rajendra G. Mehta,Richard C. Moon,John M. Pezzuto +11 more
TL;DR: It is suggested that resveratrol, a common constituent of the human diet, merits investigation as a potential cancer chemopreventive agent in humans.
Journal ArticleDOI
Caspases: killer proteases
TL;DR: Caspases (cysteinyl aspartate-specific proteinases) mediate highly specific proteolytic cleavage events in dying cells, which collectively manifest the apoptotic phenotype.
Journal ArticleDOI
SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase
Brydon L. Bennett,Dennis T. Sasaki,Brion W. Murray,Eoin C. O'leary,Steve T. Sakata,Weiming Xu,Jim C. Leisten,Aparna Motiwala,Steve Pierce,Yoshitaka Satoh,Shripad S. Bhagwat,Anthony M. Manning,David W. Anderson +12 more
TL;DR: SP600125 blocked (bacterial) lipopolysaccharide-induced expression of tumor necrosis factor-α and inhibited anti-CD3-induced apoptosis of CD4+ CD8+ thymocytes and supports targeting JNK as an important strategy in inflammatory disease, apoptotic cell death, and cancer.