Journal ArticleDOI
Inhibition of the adhesion of Entamoeba histolytica trophozoites to human erythrocytes by carbohydrates.
Reads0
Chats0
TLDR
It is concluded that E. histolytica adhesion depends on amebal metabolic energy generated from Glc and on several surface components of RBC, some of which are inactivated with glutaraldehyde whereas others are inhibited by sugars containing Gal, GlcNAc, or GalNAc residues.Abstract:
We have analyzed the effect of 14 carbohydrates (seven monosaccharides, four disaccharides and three aminosugars) on the adhesion of Entamoeba histolytica HK9 trophozoites to human red blood cells (RBC). Amebal adhesion was significantly inhibited by five of these carbohydrates with the following order of potency: lactose (Lac) greater than N-acetylgalactosamine (GalNac) greater than melibiose (Mel) greater than galactose (Gal) greater than N-acetylglucosamine (GlcNAc). The mean inhibitory concentration of Lac was 2.66 mM. Adhesion increased by 20% in the presence of 5.5 mM glucose (Glc). Inhibition of the adhesion was lower in the absence rather than in the presence of Glc only with Gal-NAc, whereas it was similar with Lac, Mel, Gal, and GlcNAc in both cases. The initial rate of amebal adhesion decreased 27% by RBC fixation, but adhesion to fixed RBC was also inhibited by the same five carbohydrates. Inhibition was higher in mixtures containing Lac, GalNAc, and Mel, than with the same isolated carbohydrates; Lac + Gal-NAc was the most potent mixture. Inhibition decreased when Lac, GalNAc, and Mel were mixed either with Gal or GlcNAc. We conclude that E. histolytica adhesion depends on amebal metabolic energy generated from Glc and on several surface components of RBC, some of which are inactivated with glutaraldehyde whereas others are inhibited by sugars containing Gal, GlcNAc, or Gal-NAc residues.read more
Citations
More filters
Journal ArticleDOI
Human milk oligosaccharides: Every baby needs a sugar mama
TL;DR: An accumulating body of evidence suggests that HMOs are antiadhesive antimicrobials that serve as soluble decoy receptors, prevent pathogen attachment to infant mucosal surfaces and lower the risk for viral, bacterial and protozoan parasite infections.
Journal ArticleDOI
The functional biology of human milk oligosaccharides.
TL;DR: Current knowledge as well as future challenges and opportunities related to the functional biology of HMOs are reviewed, with potential benefits for the neonate.
Journal ArticleDOI
Structure-Function Relationships of Human Milk Oligosaccharides
Lars Bode,Evelyn Jantscher-Krenn +1 more
TL;DR: Examples of how the structural characteristics of these human milk oligosaccharides determine functionality are provided, and intervention studies are needed to confirm that the structure-specific effects observed at the laboratory bench translate into benefits for the human infant.
Journal ArticleDOI
Human milk oligosaccharides reduce Entamoeba histolytica attachment and cytotoxicity in vitro.
Evelyn Jantscher-Krenn,Tineke Lauwaet,Laura A. Bliss,Sharon L. Reed,Frances D. Gillin,Lars Bode +5 more
TL;DR: In vitro results show that physiological concentrations of isolated, pooled HMO detach E. histolytica attachment and cytotoxicity, and Galacto-oligosaccharides (GOS), which also contain terminal Gal and are currently added to infant formula to mimic some of the beneficial effects of HMO, completely abolished E. Histolytico-induced destruction of human intestinal epithelial HT-29 cells in a dose-dependent manner.
Journal ArticleDOI
High affinity binding of the Entamoeba histolytica lectin to polyvalent N-acetylgalactosaminides.
TL;DR: The data support a model in which a unique pattern of spaced multiple GalNAc residues are the highest affinity targets for the E. histolytica lectin, and suggest that synthetic saccharide-derivatized linear polymers were the most potent.
References
More filters
Journal ArticleDOI
Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors
TL;DR: Data is presented indicating that attachment of Escherichia coli to epithelial cells is mediated by mannose (orMannose-like) receptors present on the surface of the latter, indicating an important step in the infectious process.
Journal ArticleDOI
Hemagglutination of Human Group A Erythrocytes by Enterotoxigenic Escherichia coli Isolated from Adults with Diarrhea: Correlation with Colonization Factor
TL;DR: The mannose-resistant hemagglutinin of ETEC was found to possess many characteristics previously associated with CFA, which is a surface-associated fimbriate heatlabile antigen, and the functionally and morphologically similar K88 and K99 antigens of animal-specific ETEC.
Journal ArticleDOI
Techniques of axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae.
Journal ArticleDOI
Role of Adherence in Cytopathogenic Mechanisms of Entamoeba Histolytica: STUDY WITH MAMMALIAN TISSUE CULTURE CELLS AND HUMAN ERYTHROCYTES
TL;DR: Findings indicate that the adherence of E. histolytica to target cells requires microfilament function, is via a specific amebic receptor that has affinity for GALNAc, and is required to lyse cells.
Journal ArticleDOI
Surface Components and Cell Recognition
William A. Frazier,Luis Glaser +1 more
TL;DR: The objective of this study was to establish a baseline level of cell adhesion between EMTs and plasma Membrane Fractions in the Nervous System and to establish an upper bound on the total number of cells that responded to EMT treatment.
Related Papers (5)
Role of Adherence in Cytopathogenic Mechanisms of Entamoeba Histolytica: STUDY WITH MAMMALIAN TISSUE CULTURE CELLS AND HUMAN ERYTHROCYTES
Role of the galactose lectin of Entamoeba histolytica in adherence-dependent killing of mammalian cells.
L. D. Saffer,William A. Petri +1 more