Journal ArticleDOI
Interrelationships among fluorometric analyses of spermatozoal function, classical semen quality parameters and the fertility of frozen-thawed bovine spermatozoa
TLDR
Cryopreserved spermatozoa from 8 bulls were used and multiple regression equations indicated that combinations of semen quality measurements could be useful in estimating fertilizing potential.About:
This article is published in Theriogenology.The article was published on 1993-05-01. It has received 105 citations till now. The article focuses on the topics: Semen quality & Sperm motility.read more
Citations
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Journal ArticleDOI
Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.
D. L. Garner,Larry Johnson +1 more
TL;DR: The proportion of living sperm in semen from six representative mammals was assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI), a newly developed fluorescent nucleic acid stain that stained the nuclei ofliving sperm bright green as determined by simultaneous examination of fluorescence and motility.
Journal ArticleDOI
Laboratory semen assessment and prediction of fertility: still utopia?
TL;DR: Strategies for the discovery of in vitro predictors of semen fertility require evaluations of low sperm doses for AI, so that differences in innate in vivo fertility can be accurately detected.
Journal ArticleDOI
The role of mitochondria in energy production for human sperm motility.
TL;DR: The present study gives an overview of the current knowledge on the energy-producing metabolic pathways operating inside human sperm mitochondria and critically analyse the differences with respect to somatic mitochondria.
Journal ArticleDOI
Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility
TL;DR: CASA, with high repeatability, provided a more discriminating estimate of the percentage of motile sperm cells than did the subjective procedure, and bull effect was much greater than any other variable in the experiments.
Journal ArticleDOI
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide.
TL;DR: The combination of SYBR-14 and PI was determined to be an effective tool for assessing the viability of fresh or cryopreserved sperm.
References
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Journal ArticleDOI
Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry.
TL;DR: Mitochondrial function, measured by rhodamine 123 (R123) fluorescence, was depressed by the mitochondrial inhibitors rotenone (64%) or monensin (52%), establishing that mitochondrial damage can be detected.
Journal ArticleDOI
Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analyses.
TL;DR: In this paper, a fluorogenic stain consisting of carboxyfluorescin diacetate (CFDA) and the relatively membrane-impermeant nuclear stain propidium iodide (PI) was used to distinguish three distinct populations of spermatozoa.
Journal ArticleDOI
Sperm transport in the cow : peri-ovulatory redistribution of viable cells within the oviduct
R.H.F. Hunter,Ian Wilmut +1 more
TL;DR: Evidence was obtained that spermatozoa competent to penetrate the egg(s) do not pass directly to the site of fertilisation at the isthmic-ampullary junction but instead are largely sequestered before ovulation in the caudal region of theIsthmus, possibly for 18-20 hours or more.
Journal ArticleDOI
Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility.
TL;DR: This is the first report of a sperm “motility” assay based on measurements of mitochondriab membrane potential, and offers an advantage over the slower, semiquantitative methods used currently for analyses of sperm motility and viability.
Journal ArticleDOI
Changes in deoxyribonucleoprotein during spermiogenesis in the bull
TL;DR: In this paper, the changes in deoxyribonucleoprotein during spermiogenesis in the bull were studied by the use of quantitative cytochemical methods, which indicated an alteration of the spermatid histone into a more basic arginine-rich protein.
Related Papers (5)
Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.
D. L. Garner,Larry Johnson +1 more