Journal ArticleDOI
Interspecific protoplast fusion in fission yeasts
TLDR
Protoplasts of auxotrophic mutants isolated from Schizosaccharomyces pombe and octosporus were fused, and their asymmetric segregation indicated thatSchizosACCharomyces octospora might be dominant in the fused cells.Abstract:
Protoplasts of auxotrophic mutants isolated fromSchizosaccharomyces pombe andSchizosaccharomyces octosporus were fused. The fusion products were capable of complementation and growth on minimal medium, but the morphological observations suggested a disturbed physiological balance, which was reflected in low viability, osmotic sensitivity, and the formation of incomplete cell wall. Their asymmetric segregation indicated thatSchizosaccharomyces octosporus might be dominant in the fused cells.read more
Citations
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Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts
Lucia Morales,Bernard Dujon +1 more
TL;DR: Problems of meiotic fertility, ploidy constraint, gene and gene product compatibility, and nucleomitochondrial interactions are discussed and placed in the context of other known mechanisms of yeast genome evolution as a model for eukaryotes.
Journal ArticleDOI
Phylogenesis of fission yeasts. Contradictions surrounding the origin of a century old genus
TL;DR: The phylogenesis of fungi is controversial due to their simple morphology and poor fossilization, but traditional classification supported by morphological studies and physiological traits placed the fission yeasts in one group with ascomycetous yeasts, however, the rRNA sequence comparisions revealed an enormous evolutionary gap between Saccharomyces and Schizosac charomyces.
Journal ArticleDOI
The use of mitochondrial mutants in the isolation of hybrids involving industrial yeast strains.
TL;DR: In this paper, methods for the isolation of hybrids in which one or both of the parental strains are industrial yeasts, using mitochondrial mutations as markers for the selection and isolation of the hybrids, are described.
Journal ArticleDOI
Schizosaccharomyces cryophilus sp. nov., a new species of fission yeast.
TL;DR: Schizosaccharomyces cryophilus as mentioned in this paper is the closest known relative of S. octosporus, with the sequences of the two species differing by 25 nucleotide substitutions (>4%).
References
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Journal ArticleDOI
A method for high-frequency intergeneric fusion of plant protoplasts.
K. N. Kao,M. R. Michayluk +1 more
TL;DR: None of the protoplasts from pea and barley leaves, either with or without PEG treatment, underwent cell division during the period of observation, and the mechanism of adhesion and fusion of theprotoplasts has been discussed.
Journal ArticleDOI
Mechanisms of cell fusion.
TL;DR: These observations are consistent with Poste and Allison's idea that the aggregation of intrinsic membrane proteins is important in membrane fusion but it is suggested that fusion may occur in regions of perturbed lipid bilayer which are thus denuded of intramembranous particles.
Journal ArticleDOI
Fusion of yeast spheroplasts.
TL;DR: Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions and the resulting zygotes were shown to be diploid cells of matingtype aa.
Journal ArticleDOI
Fusion of bacterial protoplasts
TL;DR: It is concluded that the observed formation of prototropic bacteria is due to protoplast fusion, a process which does not induce prophage development, and that the only stable products of the resulting diploid state are haploid recombinants.
Journal ArticleDOI
Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type.
Matthias Sipiczki,L. Ferenczy +1 more
TL;DR: Cytological and haploidization examinations showed that the fusion cells are not heterokaryons, and that the increased amount of genetic material is situated in one nucleus.
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Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type.
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