Journal ArticleDOI
Fungal protoplasts: isolation, reversion, and fusion
TLDR
The protoplast Fusion and the Hybridization of Fungal Species and Transformation in Fungi by Using Protoplasts by using Enzymatic and Nonenzymatic methods are studied.Abstract:
INTRODUCTION 21 ISOLATION OF PROTOPLASTS AND THEIR P ROPERTIES 22 Enzymatic Methods for Protoplast Isolation 22 Nonenzymatic Procedures for Protoplast Release 26 Properties of Protopiasts 27 WALL REGENERATION AND REVERSION OF PROTOPLASTS 28 Frequency of Protoplast Reversion 28 Morphology of Protoplast Reversion 29 Ultrastructure and Composition of the Regenerated Wall ..... 30 Biochemical Aspects oj Wall Biogenesis 31 PROTOPLAST FUSION AND GENETIC MANIPULATION 33 Protoplast Fusion and the Hybridization of Fungal Species 33 Transformation in Fungi by Using Protoplasts . .. ........ 36 CONCLUDING REMARKS 36read more
Citations
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Enzymology and Molecular Biology of Aflatoxin Biosynthesis.
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Menacing mold: the molecular biology of Aspergillus fumigatus.
Axel A. Brakhage,Kim Langfelder +1 more
TL;DR: This review summarizes the current knowledge on the biology, molecular genetics, and genomics of A. fumigatus and has a major impact on the development of novel strategies leading to the identification of antimycotic drugs.
Journal ArticleDOI
Efficient protoplast isolation from fungi using commercial enzymes
Paul F. Hamlyn,Rosemary E. Bradshaw,Fiona M. Mellon,Claro M. Santiago,Jane M. Wilson,John F. Peberdy +5 more
TL;DR: It is shown that high yields of protoplasts can be prepared from a variety of fungi using relatively cheap commercial enzymes.
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Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts
Lucia Morales,Bernard Dujon +1 more
TL;DR: Problems of meiotic fertility, ploidy constraint, gene and gene product compatibility, and nucleomitochondrial interactions are discussed and placed in the context of other known mechanisms of yeast genome evolution as a model for eukaryotes.
Journal ArticleDOI
Genetic Engineering of Filamentous Fungi
TL;DR: Several fungal species have biological properties and genetic systems that make them ideally suited for basic biological research, and recently developed techniques for genetic engineering of filamentous fungi make it possible to alter their detrimental and beneficial activities in novel ways.
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