Journal ArticleDOI
Ionophore A23187 induces acrosome reactions in sea urchin and guinea pig spermatozoa.
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TLDR
Ionophore A23187, in the presence of extracellular Ca+2, induces a morphologically normal acrosome reaction in sperm of the sea urchin and precocious acrosomes reaction and activation of guinea pig sperm.Abstract:
Ionophore A23187, in the presence of extracellular Ca+2, induces a morphologically normal acrosome reaction in sperm of the sea urchin and precocious acrosome reaction and activation of guinea pig sperm. Increased membrane permeability to Ca+2 is responsible for initiating the acrosome reaction in both sea urchin and guinea pig sperm. In sea urchin sperm, the permeability change is brought about by egg jelly, whereas in the guinea pig sperm it accompanies capacitation.read more
Citations
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Book ChapterDOI
Mechanisms of Fertilization in Mammals
TL;DR: This chapter will discuss how mammalian spermatozoa prepare themselves for fertilization and how the spermatozosa and eggs interact during fertilization.
Journal ArticleDOI
A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa
TL;DR: The model illustrates enzymatic reactions which are believed to regulate the permeability and integrity of the plasma membrane overlying the acrosome during interactions between the male gamete and capacitation factors present in fluids of the female genital tract.
Journal ArticleDOI
Mouse gamete interactions during fertilization in vitro. Chlortetracycline as a fluorescent probe for the mouse sperm acrosome reaction.
P M Saling,B T Storey +1 more
TL;DR: It is concluded that the plasma membrane of the mouse sperm is responsible for recognition of the egg's zona pellucida and that the obligatory sequence of reactions leading to fusion of mouse gametes is binding of the intact sperm to the zonae peLLucida, followed by the acrosome reaction at theZona surface, followed in turn by sperm penetration of the zona.
Book ChapterDOI
Mechanisms of activation of sperm and egg during fertilization of sea urchin gametes.
TL;DR: The chapter describes the important roles played by intracellular calcium and cytoplasmic pH in turning on the cell metabolism and the consequences of sperm fusion with the egg that leads to the responses that result in the activation of embryonic development.
Journal ArticleDOI
An influx of extracellular calcium is required for initiation of the human sperm acrosome reaction induced by human follicular fluid.
Paul Thomas,Stanley Meizel +1 more
TL;DR: It is shown that an influx of extracellular Ca2+ is responsible for initiating the acrosome reaction in human sperm treated with human follicular fluid, and this is the first demonstration in mammalian sperm that a potentially physiological stimulus can cause an increase in intracellularCa2+ concomitant with the acosome reaction.
References
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Journal ArticleDOI
A23187: a divalent cation ionophore.
Peter W. Reed,Henry A. Lardy +1 more
TL;DR: It is proposed that a cyclic, energy-dissipating flux of mitochondrial calcium accounts for uncoupling by A23187, whereas inhibition of ATPase and glutamate oxidation and increased potassium permeability of mitochondria result from antibiotic-mediated magnesium efflux.
Journal ArticleDOI
Calcium dependence of the acrosome reaction and activation of guinea pig spermatozoa.
Ryuzo Yanagimachi,Noriko Usui +1 more
TL;DR: Guinea pig spermatozoa fail to fertilize eggs in Ca 2+ -free media primarily because of specific inhibition of the acrosome reaction and activation of the spermatoza, and possible mechanisms by which Ca 2+, a nonvolatile substance, triggers these processes are discussed.
Journal ArticleDOI
Is calcium ionophore a universal activator for unfertilised eggs
TL;DR: It is found that calcium ionophore activation is common to several species widely separated phylogenetically and has not been obtained with Acmaea and Ciona eggs by other methods.
Journal ArticleDOI
Calcium-induced extrusion of secretory granules (exocytosis) in mast cells exposed to 48-80 or the ionophores A-23187 and X-537A.
D. E. Cochrane,W. W. Douglas +1 more
TL;DR: Support is considered for the view that Ca influx mediates stimulus-secretion coupling and does so by initiating exocytosis, and responses seem independent of possible shifts in the alkali metal ions.
Journal ArticleDOI
Studies on the acrosome. iii. effect of calcium deficiency
TL;DR: After reversal of agglutination, sea urchin spermatozoa show no reduction in fertilizing capacity when used to inseminate unfertilized eggs in sea water, in marked contrast to the se...
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Calcium dependence of the acrosome reaction and activation of guinea pig spermatozoa.
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