Journal ArticleDOI
Isolation and characterization of a marine methanogenic bacterium from the biofilm of a shiphull in Los Angeles harbor
Raj Boopathy,Lacy Daniels +1 more
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A marine mesophilic, irregular coccoid methanogen, which shows close resemblance to Methanococcus sp.Abstract:
A marine mesophilic, irregular coccoid methanogen, which shows close resemblance toMethanococcus sp., was isolated from the biofilm of shiphulls docked in Los Angeles harbor. Hydrogen plus carbon dioxide or formate served as substrates for methanogenesis in a mineral salt medium. The isolate did not use acetate and methanol as sole source of carbon and energy. The organism had an optimal pH range of 6.8–7.0 and a temperature optimum of 37°C. Elevated levels of sodium chloride were required for optimum growth. Optimum levels of total sulfide and magnesium chloride for growth were 1.0mm and 10mm respectively. The isolate used ammonia as nitrogen source. The concentration of 30mm ammonium chloride supported maximum growth of the isolate.read more
Citations
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Modeling substrate inhibition of microbial growth.
TL;DR: A graphical method existing in enzyme kinetics is successfully adapted and further developed to determine the number of inhibition sites of a basic functional unit of a bacterial cell.
Journal ArticleDOI
Isolation and characterization of a methanogenic bacterium from swine manure
TL;DR: A mesophilic, Gram positive, irregular coccoid methanogen, which showed close resemblance to a Methanosarcina sp.
Journal ArticleDOI
Mathematical Modelling of Bioethanol Fermentation From Glucose, Xylose or Their Combination – A Review
Journal ArticleDOI
Quantitative analysis of acetate oxidation in the presence of iron in a thermophilic methanogenic reactor
TL;DR: Experimental results showed that goethite and hematite promoted the methane production and generation rate and partially relieved the negative effects of H2, and theoretical calculations revealed that the rates of iron oxide mediated interspecies electron transfer among syntrophic partners were 105-106 times higher than those attainable via interspecies H2 transfer.
13 The Family Methanococcaceae
TL;DR: This family of mesophilic or thermophilic and neutrophilic methanogens belongs to the order Methanococcales, and members are coccoid in shape, motile, and have a generation time of less than 3 h under optimal conditions.
References
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Journal ArticleDOI
New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere.
W E Balch,R S Wolfe +1 more
TL;DR: A very sensitive and precise requirement for HS-CoM in the nutrition of this fastidious anaerobe is revealed.
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Limits of the Natural Environment in Terms of pH and Oxidation-Reduction Potentials
TL;DR: In this article, the electron and the proton content (measured as electrode potential [Eh] and pH) of an environment characterize this environment in many ways, and the electrode potential and the pH are used as empirical parameters rather than as electrochemical data capable of thermodynamic interpretation.
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The atmospheric cycle of methane
TL;DR: The atmospheric distribution of CH 4 is rather uniform zonally and vertically but exhibits a slight gradient with latitude in the troposphere; in the stratosphere it shows a definite decrease with altitude as discussed by the authors.
Journal ArticleDOI
Methanococcus thermolithotrophicus, a novel thermophilic lithotrophic methanogen
TL;DR: An autotrophic thermophilic motile coccoid methanogen was isolated from geothermally heated sea sediments close to Naples, Italy and is clearly defined to be a member of the genus Methanococcus.
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Isolation and characterization of Methanobacterium ruminantium N. sp.
Paul H. Smith,R. E. Hungate +1 more
TL;DR: An attempt is made to determine the numbers of methanogenic bacteria in bovine rumen contents and to isolate the predominant types in pure culture.