Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci.
Karen M. Léon-Kloosterziel,Marta Alvarez Gil,Gerda J. Ruijs,Steven E. Jacobsen,Neil E. Olszewski,Steven H. Schwartz,Jan A. D. Zeevaart,Maarten Koornneef +7 more
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TLDR
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype.Abstract:
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.read more
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