Journal ArticleDOI
Isolation of rough and smooth microsomes from rat liver by means of a commercially available centrifuge
Anders Bergstrand,Gustav Dallner +1 more
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It is pointed out in this paper that a high cross-contamination occurs when fractionation is performed with these Spinco rotors, however, good separation with a high yield can be obtained with the Spinco centrifuge if the 40.2 rotor and the procedure that is described are employed.About:
This article is published in Analytical Biochemistry.The article was published on 1969-06-01. It has received 81 citations till now. The article focuses on the topics: Centrifuge.read more
Citations
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Journal ArticleDOI
Switch-like Control of SREBP-2 Transport Triggered by Small Changes in ER Cholesterol: A Delicate Balance
TL;DR: Using a new method to purify ER membranes from cultured cells, it is shown that Scap responds cooperatively to ER cholesterol levels, creating a sensitive switch that controls the cholesterol composition of cell membranes with remarkable precision.
Journal ArticleDOI
Modulation of the bilayer thickness of exocytic pathway membranes by membrane proteins rather than cholesterol
Kakoli Mitra,Iban Ubarretxena-Belandia,Iban Ubarretxena-Belandia,Tomohiko Taguchi,Graham Warren,Donald M. Engelman +5 more
TL;DR: Protein-depleted membranes demonstrated changes in thickness of up to 5 Å, suggesting that (i) membrane proteins rather than cholesterol modulate the average bilayer thickness of eukaryotic cell membranes, and (ii) proteins and lipids are not naturally hydrophobically matched in some biological membranes.
Journal ArticleDOI
Methods of determining plasma and tissue binding of drugs. Pharmacokinetic consequences.
Gian Maria Pacifici,A. Viani +1 more
TL;DR: A comparison of different plasma protein binding techniques is made which shows that the size of the unbound fraction of drug may be influenced by the method used, and Mathematical and graphical methods to compute the pharmacokinetic parameters are discussed.
Journal ArticleDOI
Subcellular distribution of a mammalian hepatic binding protein specific for asialoglycoproteins.
W E Pricer,G Ashwell +1 more
TL;DR: Evidence is presented to support the identification of a unique membrane binding protein in a variety of subcellular organelles including the Golgi complex, the lysosomes, the smooth microsomes, and the external plasma membranes prepared from rat liver homogenates.
References
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Journal ArticleDOI
Liver microsomes; an integrated morphological and biochemical study.
TL;DR: Rat liver, liver homogenates, and microsome fractions separated therefrom were examined systematically in the electron microscope in sections of OsO 4 -fixed, methacrylate-embedded tissue and pellets and it was found that most microsomes are morphologically identical with the rough surfaced elements of the endoplasmic reticula of hepatic cells.
Journal ArticleDOI
BIOGENESIS OF ENDOPLASMIC RETICULUM MEMBRANES : II. Synthesis of Constitutive Microsomal Enzymes in Developing Rat Hepatocyte
TL;DR: The results suggest that during development the enzymic pattern is not influenced by the fatty acid or phospholipid composition of ER membranes, and the enzymes are synthesized in the rough part, then transferred to the smooth part, of the ER.
Journal ArticleDOI
BIOGENESIS OF ENDOPLASMIC RETICULUM MEMBRANES I. Structural and Chemical Differentiation in Developing Rat Hepatocyte
TL;DR: Results strongly suggest that new membrane is synthesized in the rough ER and subsequently transferred to the smooth ER in rat hepatocytes during a period of rapid cell differentiation.
Journal ArticleDOI
Lipid composition and turnover of rough and smooth microsomal membranes in rat liver
Hans Glaumann,Gustav Dallner +1 more
TL;DR: The membranes of the rough and the two smooth microsomal subfractions from rat liver have a similar phospholipid composition, but are dissimilar in their neutral lipid content and in the incorporation rate of precursors into membrane lipids.
Journal ArticleDOI
Biosynthesis of Glycoprotein by Liver THE INCORPORATION IN VIVO OF 14C-GLUCOSAMINE INTO PROTEIN-BOUND HEXOSAMINE AND SIALIC ACID OF RAT LIVER SUBCELLULAR FRACTIONS
G. Ross Lawford,Harry Schachter +1 more
TL;DR: The conclusion is drawn that hexosamine is first incorporated into newly synthesized polypeptide while it is still attached to the ribosome on which it is being assembled and that further Hexosamine residues are incorporated after detachment from the ribOSome as the polypePTide traverses the channels of the rough and smooth surfaced endoplasmic reticulum on its way out of the cell into the plasma.
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The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more