Showing papers in "Journal of Lipid Research in 1968"

TL;DR: Several indications exist that the acyltransferase reaction is the major source of plasma esterified cholesterol in man, and one possibility is that it plays a role in the transport of cholesterol from peripheral tissues to the liver.

TL;DR: The clearcut separation of lipoproteins by this method will facilitate the classification of hyperlipoproteinemias and improve quantitative estimates oflipoprotein distribution.

TL;DR: Four methods for the sizing of adipose cells in small samples of human or animal adipose tissue are compared and the use of the Coulter counter to count cells in a suspension derived from a known amount of tissue and subjected to osmium tetroxide fixation is recommended.

TL;DR: Brain inositol phosphoglycerides, the fatty acid composition of which has not been studied systematically before, were characterized by a large concentration of arachidonate which was nearly as high for white as for gray matter and showed only small changes with age.

TL;DR: The spherules constitute a valuable model system with which to study the properties of biological membranes that may be dependent on their lipid components.

TL;DR: A simplified method for the preparation of the Dittmer-Lester spray for the detection of phospholipids on thin-layer chromatograms is described.

TL;DR: A rapid, sensitive spectrophotometric method for the analysis of sulfatides is described, applicable to theAnalysis of sulfolipids in tissue extracts and gives values similar to those obtained by previously published procedures.

TL;DR: Experimental data indicate that at least four different transport mechanisms account for net movement of bile acids across the gastrointestinal tract and active transport and passive nonionic diffusion are quantitatively of the greatest importance.

TL;DR: Evidence that considerable amounts of neutral sterols are "lost" during their passage through the intestinal tract is gathered, due not to errors in stool collection or to technical errors, but to intestinal bacterial degradation of neutral 3beta-OH,Delta(5)-sterols to products not recognized as steroids in the analytical methods used.

TL;DR: Myelin phospholipids have been examined by a separation-reaction-separation procedure for two-dimensional thin-layer chromatography on silica gel and development in the second dimension quantitatively separates acid-labile and acid-stable phosphoglycerides as well as the aldehydes released from the acid- labile phosphoglycers.

TL;DR: Thin-layer chromatography for the separation of mono-, di-, and triphosphoinositides (0.3-3 micro g total phosphorus) is described.

TL;DR: The results of these studies are in accord with the concept that the presence of a Delta(5)-double bond is not required for the enzymatic formation of the 7alpha-hydroxy derivative.

TL;DR: The membranes of the rough and the two smooth microsomal subfractions from rat liver have a similar phospholipid composition, but are dissimilar in their neutral lipid content and in the incorporation rate of precursors into membrane lipids.

TL;DR: Fatty acid changes with age were the same for cerebrosides and sulfatide but occurred later in the sulfatides, which supports the hypothesis that the cerebroides are precursors of the sulf atides.

TL;DR: In this paper, the ability of two products of alcohol metabolism, acetate and lactate, to lower free fatty acids in man was investigated, and it was shown that acetate, a metabolite of ethanol, reduces plasma free acids at plasma acetate levels comparable to those resulting from ethanol metabolism.

TL;DR: It has been demonstrated that, in spite of the omission of the usual purification steps, the method is specific for bile acids.

TL;DR: It is concluded that acidic lipids are mainly responsible for the sudanophilia detected histologically at sites of new calcification.

TL;DR: Commercial serum albumin was found to contain lactate, pyruvate, and especially citrate in addition to fatty acids, andCharcoal treatment followed by prolonged dialysis was effective in removing most of these contaminants.

TL;DR: The fatty liver observed in CD rats results from an impaired release of hepatic triglycerides into plasma and considerably less radioactivity accumulated in plasma triglycerides and phospholipids of CD rats than of CS animals.

TL;DR: Intestinal transit studies indicate that the distinction between sitosterol and cholesterol, when fed together, took place during the process of uptake into the intestinal mucosa, and the rapid and complete absorption of the triglyceride fat and the subsequent transit of the intestinal content to the large intestine are most probably important factors in the determination of the extent of absorption of nonglyceride fat.

TL;DR: Phytol-U-(14)C was adsorbed on algae and ingested in this form by zooplanktonic copepods (two species of Calanus) and is interpreted as a likely biological source of pristane in nature.

TL;DR: The results support the possibility that the acyltransferase indirectly increases the concentration of very low density lipoprotein cholesteryl esters in vivo.

TL;DR: The detailed relationship between the relative composition of the potential precursor acids, the esterification rates of their CoA thiol ester derivatives, and the relative compositions of the fatty acids in the product, lecithin, suggests that in humans the stromal acyltransferases could be the significant enzymatic factor controlling the fatty acid composition at the 2-position in erythrocytes.

TL;DR: The hypothesis that the short-chain fatty acids are esterified with long-chain diglycerides, or are substituted in glycerophosphatide intermediates, during the final step in the biosynthesis of milk fat triglycerides is supported.

TL;DR: The use of a stable solution of ferric chloride for the detection of cholesterol and cholesteryl esters on thin-layer chromatographic layers is described and the levels of sensitivity for the new reagent are shown.

TL;DR: A comparison has been made of the milk and adipose tissue triglycerides of rabbits and guinea pigs provided with one diet and of rats and mice provided with another, and the significance of enzymes that synthesize short-chain fatty acids in mammary gland is discussed.

TL;DR: C(20) and C(22) polyunsaturated acids were confined almost exclusively to the 2-position of these lipids, where they comprised the majority of 2-substituents in EGP and SGP (oleic acid predominated in this position in CGP).

TL;DR: The studies suggest that the intracellular concentration of newly esterified triglyceride and (or) some other lipid metabolite can specifically influence the release and perhaps the synthesis of d < 1.007 lipoprotein protein and the liver increases its rate of triglyceride release primarily by producing more lipop protein particles.

TL;DR: Electron microscopy showed a strong morphological resemblance between the white layer and alveolar lining material in situ and the composition of the lipid portion was the same in three dogs.

TL;DR: A comparison of several species indicates that a much greater proportion of fetal fatty acids comes from the mother in the guinea pig and rabbit than in the rat, the sheep, or man.