Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants
Richard Wells,Max L. Birnstiel +1 more
TLDR
From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.Abstract:
1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1.706g.cm.(-3), whereas chloroplastal DNA has a buoyant density of 1.697g.cm.(-3). 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distribution of the nuclear DNA and is not resolved as a satellite. 3. Both chloroplastal DNA and mitochondrial DNA from lettuce renature rapidly. 4. The kinetic complexity of mitochondrial DNA is > 10(8) daltons. 5. Chloroplastal DNA is made up from fast and slow renaturing sequences with kinetic complexities of 3x10(6) and 1.2x10(8) daltons respectively. 6. From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.read more
Citations
More filters
Book ChapterDOI
The ribosomal RNA cistrons.
TL;DR: The chapter presents a picture of the detailed physical structure of the ribosomal cistron and how it is integrated into the genomic DNA, both of which have important implications for cell differentiation and for evolutionary theory.
Journal ArticleDOI
Chromatid structure: relationship between DNA content and nucleotide sequence diversity.
TL;DR: This method is based on the demonstration that nucleotide sequence diversity may be estimated from the kinetics of renaturation of denatured DNA and shows that most of the nucleotide sequences in mouse, Drosophila, and Ciona DNA are present only once per sperm.
Journal ArticleDOI
Heterogeneous population of mitochondrial DNA molecules in higher plants
Francis Quetier,Fernand Vedel +1 more
TL;DR: EcoRI specific cleavage is used to evidence the general occurrence of heterogeneous population of mt-DNA molecules within an higher plant and to detect clear differences between mt-DNAs extracted from normal and cytoplasmic male-sterile wheat.
Book ChapterDOI
Molecular Biology of Higher Plant Mitochondrial DNA
D. R. Pring,D.M. Lonsdale +1 more
TL;DR: The analysis of higher plant mitochondrial DNA (mtDNA) preparations by electron microscopy reveals that the majority of the DNA molecules observed are linear, with no fixed size distributions.
Journal ArticleDOI
The Structure of Chloroplast DNA
TL;DR: This paper presents a systematic literature review of physical studies on the structure of ctDNA and its role in Kinetic Comple;city and Circularity.