Light-stimulated phosphorylation of bovine visual pigments by adenosine triphosphate.
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TLDR
The phosphorylation reaction does not appear to have greater specificity for rhodopsin than for other proteins present as minor components in retinal outer segment preparations; however, of a variety of soluble proteins added to the incubation mixture in high concentrations along with outer segment membranes, only calf thymus histone is capable of binding 32P from [γ-32P]ATP.About:
This article is published in Journal of Biological Chemistry.The article was published on 1973-01-25 and is currently open access. It has received 137 citations till now. The article focuses on the topics: Retinaldehyde & Adenosine triphosphate.read more
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Beta-adrenergic receptor kinase: identification of a novel protein kinase that phosphorylates the agonist-occupied form of the receptor
TL;DR: The existence of this novel cAMP-independent kinase, which preferentially phosphorylates the agonist-occupied form of the beta-adrenergic receptor, suggests a mechanism that may explain the homologous or agonists-specific form of adenylate cyclase desensitization and suggests a general mechanism for regulation of receptor function.
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Rhodopsin and phototransduction: a model system for G protein-linked receptors.
P A Hargrave,J H McDowell +1 more
TL;DR: These auxilliary proteins that function with rhodopsin in rod cells serve as models for understanding how other members of the receptor family may function in conjunction with other G proteins, kinases, and arrestin‐like proteins.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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An Adenosine 3',5'-Monophosphate-dependant Protein Kinase from Rabbit Skeletal Muscle
TL;DR: A protein kinase that catalyzes an adenosine 3',5'-monophosphate (cyclic AMP)-dependent phosphorylation of casein and protamine has been purified from rabbit skeletal muscle.
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Cyclic nucleotide-dependent protein kinases, iv. widespread occurrence of adenosine 3′,5′-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom
J.F. Kuo,Paul Greengard +1 more
TL;DR: The data support a unifying theory for the mechanism of action of adenosine 3',5'-monophosphate, namely that its many and diverse effects are mediated through activation of tissue-specific protein kinases.
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Two new staining procedures for quantitative estimation of proteins on electrophoretic strips.
TL;DR: Two new procedures are described for the estimation of protein by direct photometry on electrophoretic strips and the protein complexes of procion brilliant blue RS and coomassie brilliant blue R250 are shown to follow Beer's law.