Journal ArticleDOI
Location of bacterial skin flora.
TLDR
An investigation was undertaken by means of correlative light and electron microscopy the location of normal skin flora, i.e., the site where bacteria are normally found in the epidermis.Abstract:
the past 30 years knowledge of cutanef)ua bacteriology lias gradually accumulated (Marples, M. J., 1965). Yet, one main aspect of cutaneous bacterial flora has not been completely elucidated, i.e., the site where bacteria are normally found in the epidermis. An investigation was undertaken to visualize by means of correlative light and electron microscopy the location of normal skin flora. Results of this study are presented.read more
Citations
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Journal ArticleDOI
Epidemiologic Background of Hand Hygiene and Evaluation of the Most Important Agents for Scrubs and Rubs
Günter Kampf,Axel Kramer +1 more
TL;DR: Long-lasting improvement of compliance with hand hygiene protocols can be successful if an effective and accessible alcohol-based hand rub with a proven dermal tolerance and an excellent user acceptability is supplied, accompanied by education of health care workers and promotion of the use of the product.
Journal ArticleDOI
Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin.
Tiffany C. Scharschmidt,Kimberly S. Vasquez,Mariela L. Pauli,Elizabeth G. Leitner,Kevin Y. Chu,Hong-An Truong,Margaret M. Lowe,Robert Rodriguez,Niwa Ali,Zoltan Laszik,Justin L. Sonnenburg,Sarah E. Millar,Michael Rosenblum +12 more
TL;DR: HF morphogenesis, commensal microbe colonization, and local chemokine production work in concert to recruit Tregs into neonatal skin, thereby establishing this tissue Treg niche early in life.
Journal ArticleDOI
Molecular analysis of the prevalent microbiota of human male and female forehead skin compared to forearm skin and the influence of make-up.
TL;DR: To compare the bacterial diversity of two different ecological regions including human forehead, human forearm and to estimate the influence of make‐up, a large number of bacteria from both regions are compared.
Journal ArticleDOI
Anatomical location of normal skin flora.
TL;DR: Bacterial population increased in normal skin after occlusion and beneath superficial cells of the stratum corneum, and openings of hair follicles contained numerous microorganisms which were often associated with sebum.
Book ChapterDOI
Antimicrobials and the skin physiological and pathological flora.
TL;DR: While antimicrobials may cause irritant and allergic contact dermatitis, no evidence exists that the use of antimicrobial substances may change the ecology of resident bacteria on the skin thereby leading to the overgrowth of pathogenic bacteria.
References
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Journal ArticleDOI
Improvements in epoxy resin embedding methods.
TL;DR: Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.
Journal ArticleDOI
A simplified lead citrate stain for use in electron microscopy.
TL;DR: This communication reports the use of a commercially available lead citratO to eliminate the lead citrate stain in electron microscopy.
Journal ArticleDOI
Effects of Varying the Vehicle for OsO4 in Tissue Fixation
TL;DR: Differences in the quality of OsO4 fixation of tissue cells obtained with any one of the several recommended mixtures may be referable to structural or compositional variations associated with functional changes, but in most instances the differences seem more properly considered as products of fixation.
Journal ArticleDOI
Fixation and staining of fungus hyphae and host plant root tissues for electron microscopy.
TL;DR: A deuteromycetous root-infecting fungus (Pyrenochaeta terrestris) and one of its hosts (Allium cepa) were fixed in OsO 4, OsO4 vapor, KMnO4, glutaraldehyde followed by OsO3, acrolein followed byOsO4 and a glutaralde-acrolein mixture followed by osO4.