Mediated electrochemical reduction of cytochrome c and tyrosinase at perfluorosulfonated ionomer coated electrodes

TLDR: Nafion-coated glassy carbon electrodes containing the copper(II) complex prepared from the tripodal tris(pyrazol-1-ylmethyl)amine ligand are shown to effectively mediate electron transfer to cytochrome c and the copper-containing enzyme tyrosinase.

Abstract: Nafion-coated glassy carbon electrodes containing the copper(II) complex prepared from the tripodal tris(pyrazol-1-ylmethyl)amine ligand are shown to effectively mediate electron transfer to cytochrome c and the copper-containing enzyme tyrosinase. For both species, a concentration-dependent increase in cathodic current is observed at the peak potential for the mediator following addition of protein to the buffer solution containing the modified electrode. The average increase for a cytochrome c concentration of 0.19 mM is 11±3.2%, while that for 5.6 μM tyrosinase is 9.5±4.6%