Metabolic effects of manganese deficiency in Aspergillus niger: evidence for increased protein degradation

TLDR: The effect of manganese deficiency on macromolecule synthesis has been studied in a citric acid producing strain of Aspergillus niger and addition of inhibitors of RNA, DNA or protein synthesis revealed that only emetine and cycloheximide successfully antagonized the adverse effect ofManganese ions oncitric acid accumulation.

Abstract: The effect of manganese deficiency on macromolecule synthesis has been studied in a citric acid producing strain of Aspergillus niger: pulse labelling experiments showed that the synthesis of both protein and RNA was not influenced by the presence of manganese; however, increased protein degradation occurred under manganese deficiency. This was also reflected by the increased activity of an intracellular proteinase activity under these conditions. In replacement cultures addition of inhibitors of RNA, DNA or protein synthesis revealed that only emetine and cycloheximide (which both act at the ribosome) successfully antagonized the adverse effect of manganese ions on citric acid accumulation. Manganese deficiency was also characterized by a decreased portion of polysomes and 80 S ribosomes.