Methods for evaluating the acrosomal status of mammalian sperm.
Nicholas L. Cross,Stanley Meizel +1 more
TLDR
A full understanding of the acrosome reaction is central to understanding sperm function and the existing methods and the criteria that should be considered in the choice of an assay are reviewed.Abstract:
A full understanding of the acrosome reaction is central to understanding sperm function. Acrosomal status can be determined on living, motile sperm in only a few mammalian species. For other species, many light microscopic methods have been developed, including colored stains for bright-field microscopy, and probes for fluorescence microscopy. We review the existing methods and the criteria that should be considered in the choice of an assay.read more
Citations
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Human sperm hyperactivation and capacitation as parts of an oxidative process.
de Lamirande E,Claude Gagnon +1 more
TL;DR: The data suggest that spermatozoa need a sustained O2.- generation to maintain HA and proceed to capacitation, and hypothesize that FCSu or the O2.' generated by X + XO + cat activate enzymes, possibly a reduced nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidase at the level of sperm membrane.
Journal ArticleDOI
Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry.
TL;DR: Mitochondrial function, measured by rhodamine 123 (R123) fluorescence, was depressed by the mitochondrial inhibitors rotenone (64%) or monensin (52%), establishing that mitochondrial damage can be detected.
Journal ArticleDOI
A positive role for the superoxide anion in triggering hyperactivation and capacitation of human spermatozoa
E. de Lamirande,Claude Gagnon +1 more
TL;DR: The results suggest that the superoxide anion may be involved in capacitation and hyperactivation of human spermatozoa.
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Fertilization: a sperm’s journey to and interaction with the oocyte
TL;DR: The results indicate that what has been observed in in vitro fertilization (IVF) differs significantly from what occurs during "physiological" fertilization.
Journal ArticleDOI
A test of the human sperm acrosome reaction following ionophore challenge. Relationship to fertility and other seminal parameters.
TL;DR: The test was shown to have a predictive value for fertility comparable to that of the hamster ovum sperm penetration assay and to be a simple and cost-effective addition to existing semenology.
References
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Journal ArticleDOI
Spermiogenesis of rat, mouse, hamster and guinea pig as revealed by the periodic acid-fuchsin sulfurous acid technique.
C. P. Leblond,Yves Clermont +1 more
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Two simple methods for detecting acrosome‐reacted human sperm
TL;DR: Two methods for detecting acrosome reactions of human sperm at the light microscopic level are described, rapid, give similar results, and detect an increase in acrosomes reactions following exposure to the ionophore A23187.
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Sperm capacitation and fertilization in mammals.
TL;DR: In several species evidence indicates capacitation enables sperm to penetrate the cumulus oophorus and the zona pellucida, and the question of the need for capacitation in man is as of yet resolved.
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Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay.
Cynthia R. Ward,Bayard T. Storey +1 more
TL;DR: The results imply that spermatozoa showing CTC fluorescence pattern B can be considered to be capacitated and that a functional definition for capacitation is the acquired ability to undergo the acrosome reaction rapidly when treated with acid-solubilized zonae pellucidae.
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Mouse gamete interactions during fertilization in vitro. Chlortetracycline as a fluorescent probe for the mouse sperm acrosome reaction.
P M Saling,B T Storey +1 more
TL;DR: It is concluded that the plasma membrane of the mouse sperm is responsible for recognition of the egg's zona pellucida and that the obligatory sequence of reactions leading to fusion of mouse gametes is binding of the intact sperm to the zonae peLLucida, followed by the acrosome reaction at theZona surface, followed in turn by sperm penetration of the zona.