M
Masahito Ikawa
Researcher at Osaka University
Publications - 395
Citations - 26329
Masahito Ikawa is an academic researcher from Osaka University. The author has contributed to research in topics: Sperm & Gene. The author has an hindex of 74, co-authored 338 publications receiving 22971 citations. Previous affiliations of Masahito Ikawa include Kobe University & University of Tokyo.
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Journal ArticleDOI
Green mice' as a source of ubiquitous green cells
TL;DR: Transgenic mouse lines with an ‘enhanced’ GFP (EGFP) cDNA under the control of a chicken beta‐actin promoter and cytomegalovirus enhancer were produced and all of the tissues from these transgenic lines, with the exception of erythrocytes and hair, were green under excitation light.
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DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes
Satomi Kuramochi-Miyagawa,Toshiaki Watanabe,Kengo Gotoh,Yasushi Totoki,Atsushi Toyoda,Masahito Ikawa,Noriko Asada,Kanako Kojima,Yuka Yamaguchi,Takashi W. Ijiri,Kenichiro Hata,En Li,Yoichi Matsuda,Tohru Kimura,Masaru Okabe,Yoshiyuki Sakaki,Hiroyuki Sasaki,Toru Nakano,Toru Nakano +18 more
TL;DR: In this paper, DNA methylation and Piwi-interacting small RNA (piRNA) expression were analyzed in wild-type, MILI-null, and MIWI2-null male fetal germ cells.
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Mili, a mammalian member of piwi family gene, is essential for spermatogenesis.
Satomi Kuramochi-Miyagawa,Tohru Kimura,Takashi W. Ijiri,Taku Isobe,Noriko Asada,Yukiko Fujita,Masahito Ikawa,Naomi Iwai,Masaru Okabe,Wei Deng,Haifan Lin,Yoichi Matsuda,Toru Nakano +12 more
TL;DR: The similarities in the phenotypes of the MILI- and MVH-deficient mice and in the physical binding properties of MILI and MVh indicate a functional association of these proteins in post-transcriptional regulation, and indicate that MILI is essential for the differentiation of spermatocytes.
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The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs
TL;DR: By using a fusion-inhibiting monoclonal antibody and gene cloning, a mouse sperm fusion-related antigen is identified and it is shown that the antigen is a novel immunoglobulin superfamily protein.
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Engineered CRISPR-Cas9 nuclease with expanded targeting space
Hiroshi Nishimasu,Xi Shi,Xi Shi,Soh Ishiguro,Soh Ishiguro,Linyi Gao,Linyi Gao,Seiichi Hirano,Sae Okazaki,Taichi Noda,Omar O. Abudayyeh,Omar O. Abudayyeh,Omar O. Abudayyeh,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Hideto Mori,Hideto Mori,Seiya Oura,Benjamin Ray Holmes,Benjamin Ray Holmes,Mamoru Tanaka,Motoaki Seki,Hisato Hirano,Hiroyuki Aburatani,Ryuichiro Ishitani,Masahito Ikawa,Masahito Ikawa,Nozomu Yachie,Feng Zhang,Osamu Nureki +30 more
TL;DR: A rationally engineered SpCas9 variant (SpCas9-NG) that can recognize relaxed NG PAMs is reported, which is a powerful addition to the CRISPR-Cas9 genome engineering toolbox and will be useful in a broad range of applications, from basic research to clinical therapeutics.