Methods of cell lysis and effect of detergents for the recovery of nitrile metabolizing enzyme from Amycolatopsis sp. IITR215
Vikash Babu,Bijan Choudhury +1 more
TLDR
Recovery of intracellular enzymes was enhanced by using different concentrations of sodium cholate and deoxycholate, and sorbitol proved to be an efficient lyoprotectant.About:
This article is published in Journal of Genetic Engineering and Biotechnology.The article was published on 2013-12-01 and is currently open access. It has received 7 citations till now. The article focuses on the topics: Amycolatopsis & Lysis.read more
Citations
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A roadmap for the synthesis of separation networks for the recovery of bio-based chemicals: Matching biological and process feasibility.
Kirti M. Yenkie,Wenzhao Wu,Ryan L. Clark,Brian F. Pfleger,Thatcher W. Root,Christos T. Maravelias +5 more
TL;DR: The goal is to present a roadmap for selection of appropriate technologies and generation of separation schemes for efficient recovery of bio-based chemicals by utilizing information from upstream processing, separation science and commercial requirements.
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Effects of natural deep eutectic solvents on lactic acid bacteria viability during cryopreservation.
TL;DR: It is demonstrated that NADESs can be used as green cryoprotective chemical agents (CPAs) that can efficiently increase LAB viability during cryostorage and should have great value in the food production and probiotics industries.
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Exploring a broad spectrum nitrilase from moderately halophilic bacterium Halomonas sp. IIIMB2797 isolated from saline lake.
TL;DR: The interesting feature of the study is that whole cells of Halomonas sp.
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Enhanced catalytic activity and thermal stability of 2,4-dichlorophenol hydroxylase by using microwave irradiation and imidazolium ionic liquid for 2,4-dichlorophenol removal
TL;DR: In this paper, a highly active 2,4-DCP hydroxylase was obtained and used in 2 4-dichlorophenol removal by employing microwave irradiation as a heating mode and ionic liquid (IL) as an additive.
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Amide hydrolyzing potential of amidase from halotolerant bacterium Brevibacterium sp. IIIMB2706
TL;DR: Various organic solvents compatibility profile showed that the amidase from Brevibacterium sp.
References
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Enzyme stability and stabilization—Aqueous and non-aqueous environment
TL;DR: An overview of the denaturation mechanisms in aqueous and non-aqueous environment is given in this article, and various methods of enzyme stabilization with respect to their use in the aqueously and nonaqueous environments have been given.
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Process-scale disruption of microorganisms.
TL;DR: In all cases it is essential to consider the interaction of the disruption operation with downstream units and to clearly demonstrate the cost benefits of alternative strategies.
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Disruption of Chlorella vulgaris Cells for the Release of Biodiesel-Producing Lipids: A Comparison of Grinding, Ultrasonication, Bead Milling, Enzymatic Lysis, and Microwaves
TL;DR: Overall, grinding in liquid nitrogen was identified as the most effective method in terms of disruption efficiency and time.
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Structural modifications in the peptidoglycan of Escherichia coli associated with changes in the state of growth of the culture.
TL;DR: By determining the composition in muropeptides of the murein of a number of strains of Escherichia coli, purified from cells at various states of growth, the sacculus was found to be considerably modified when cells stop active growth.