miRNA-99b-5p targets FZD8 to inhibit non-small cell lung cancer proliferation, migration and invasion.
TLDR
It is demonstrated that miR-99b-5p inhibits NSCLC cell proliferation, migration and invasion through targeting FZD8.Abstract:
Background miRNAs were found to play crucial roles in regulating cellular behaviors. The aim of this study was to investigate the biological function of miRNA-99b-5p (miR-99b-5p) in non-small cell lung cancer (NSCLC). Materials and methods miR-99b-5p expression level in NSCLC cell lines was detected by quantitative real-time PCR (qRT-PCR). Cell proliferation, migration and invasion were examined by cell counting kit-8 (CCK-8) assay, wound-healing assay and Transwell invasion assay, respectively. Dual-luciferase activity reporter assay and Western blot assay were conducted to validate the target of miR-99b-5p. Results The expression of miR-99b-5p was decreased in NSCLC cell lines compared with normal cell line. Overexpression of miR-99b-5p inhibits cell proliferation, migration and invasion in vitro. FZD8 was validated as a direct target of miR-99b-5p. Overexpression of FZD8 partially abolished the effects of miR-99b-5p mimic on NSCLC cell behaviors. Conclusion Collectively, our results demonstrated that miR-99b-5p inhibits NSCLC cell proliferation, migration and invasion through targeting FZD8. This newly identified miR-99b-5p/FZD8 axis provided novel insights into the mechanisms underlying NSCLC progression.read more
Citations
More filters
Journal ArticleDOI
Integrative Bioinformatics Approaches to Map Potential Novel Genes and Pathways Involved in Ovarian Cancer.
TL;DR: This study identified the most relevant biological networks involving DEGs that were mainly enriched in the cell cycle (in metaphase checkpoints) and revealed the role of APC in cell cycle regulation pathways.
Journal Article
Facile preparation of biocompatible nanostructured lipid carrier with ultra-small size as a tumor-penetration delivery system B Biointerfaces
TL;DR: In this article, a facile solvent diffusion method was applied to prepare an ultra-small nanostructured lipid carrier (usNLC) which was capable of encapsulating hydrophobic molecules.
Journal ArticleDOI
Non-coding RNA profile in lung cancer.
TL;DR: Recent findings about the importance of competing endogenous RNA networks in the pathogenesis of lung cancer are summarized and a list of onco-mi RNAs, tumor suppressor miRNAs, oncogenic lncRNAs and tumor suppressors based on their roles in the carcinogenic process in lung cancer is provided.
Journal ArticleDOI
MicroRNAs: Emerging oncogenic and tumor-suppressive regulators, biomarkers and therapeutic targets in lung cancer.
Shengjie Tang,Shuangjiang Li,Shuangjiang Li,Liu Tao,Yiwei He,Haiyang Hu,Zhu Yunhe,Shoujun Tang,Haining Zhou +8 more
TL;DR: In this article, a review summarizes the most recent progress on the functional mechanisms of microRNAs involved in lung cancer development and progression and further discusses the clinical application of miRNAs as putative therapeutic targets for molecular diagnosis and prognostic prediction.
Journal ArticleDOI
Silencing lncRNA DUXAP8 inhibits lung adenocarcinoma progression by targeting miR-26b-5p.
TL;DR: It is elucidated that that DUXAP8 promoted tumor progression in LUAD by targeting miR-26b-5p, which provide a novel therapeutic target for diagnosis and therapy of LUAD.
References
More filters
Journal ArticleDOI
Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries
Freddie Bray,Jacques Ferlay,Isabelle Soerjomataram,Rebecca L. Siegel,Lindsey A. Torre,Ahmedin Jemal +5 more
TL;DR: A status report on the global burden of cancer worldwide using the GLOBOCAN 2018 estimates of cancer incidence and mortality produced by the International Agency for Research on Cancer, with a focus on geographic variability across 20 world regions.
Journal ArticleDOI
microRNAs as oncogenes and tumor suppressors.
TL;DR: More than 50% of miRNA genes are located in cancer-associated genomic regions or in fragile sites, suggesting that miRNAs may play a more important role in the pathogenesis of a limited range of human cancers than previously thought.
Journal ArticleDOI
MicroRNAs as oncogenes and tumor suppressors.
TL;DR: The struggle to combat cancer — including the discovery of oncogenes, tumor suppressors, telomerase, and cancer stem cells — has revealed the complex nature of cancer in humans.
Journal ArticleDOI
miRpower: a web-tool to validate survival-associated miRNAs utilizing expression data from 2178 breast cancer patients.
András Lánczky,Ádám Nagy,Giulia Bottai,Gyöngyi Munkácsy,András Szabó,Libero Santarpia,Balázs Győrffy +6 more
TL;DR: An integrated platform capable to mine all available miRNA data to perform a survival analysis for the identification and validation of prognostic miRNA markers in breast cancer is established.
Journal ArticleDOI
Meta-analysis of human lung cancer microRNA expression profiling studies comparing cancer tissues with normal tissues
TL;DR: A meta-analysis was conducted to review the published microRNA expression profiling studies that compared the microRNAs expression profiles in lung cancer tissues with those in normal lung tissues, and the top two most consistently reported up-regulated micro RNAs were miR-210 andMiR-21.