Journal ArticleDOI
Mixture screening and mixture-amount designs to determine plant growth regulator effects on shoot regeneration from grapefruit ( Citrus paradisi macf.) epicotyls
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Zeatin riboside singly or in combination with indole-3-acetic acid resulted in the highest quality, the greatest number of explants with buds/shoots, and the greatest shoot number; and 6-benzylaminopurine and indoleAbstract:
The objective of this study was to improve shoot regeneration from grapefruit. Because many commercially grown citrus types are apomictic, important in vitro applications such as Agrobacterium-mediated transformation commonly use epicotyl explants from in vitro seedlings; thus, adequate adventitious shoot production is an important prerequisite for efficient use of these applications. Eight plant growth regulators were studied—six cytokinins (6-benzylaminopurine, kinetin, zeatin trans-isomer, 6-[γ,γ-dimethylallylamino] purine, zeatin riboside trans-isomer and meta-topolin) and two auxins (α-naphthalene acetic acid and indole-3-acetic acid). An iterative design strategy was followed that included mixture and mixture-amount experimental designs suitable for resolving proportional and concentration effects; in vitro effects of cytokinins and auxins are affected by both proportion and concentration. One-centimeter-long explants were excised from the epicotyl of etiolated, in vitro-grown seedlings. Explants were placed onto experimental formulations and cultured in growth cabinets at 27°C over 6 wk, which included 2 wk in the dark followed by 4 wk in the light. The results indicated that (1) 6-benzylaminopurine or zeatin riboside were the most effective cytokinins for inducing shoot regeneration in citrus; (2) zeatin riboside singly or in combination with indole-3-acetic acid resulted in the highest quality, the greatest number of explants with buds/shoots, and the greatest shoot number; and (3) 6-benzylaminopurine and indole-3-acetic acid improved shoot regeneration vs. 6-benzylaminopurine at a considerably lesser cost than zeatin riboside and indole-3-acetic acid.read more
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Design of experiments (DOE)—history, concepts, and relevance to in vitro culture
TL;DR: The presentation is a narrative of the historical context and the geometric basis of DOE to explain the underlying concepts and examples illustrate the use of DOE in in vitro plant culture research.
Journal ArticleDOI
Nitrogen ions and nitrogen ion proportions impact the growth of apricot (Prunus armeniaca) shoot cultures
Irina Kovalchuk,Zinat Mukhitdinova,Timur Turdiyev,Gulnara Madiyeva,Meleksen Akin,Ecevit Eyduran,Barbara M. Reed,Barbara M. Reed +7 more
TL;DR: Validation experiments comparing WPM and three trial media showed improvements in several shoot growth parameters on medium with optimized mesos and optimized nitrogen components, and suggested concentrations for an improved medium were 25 < NO3− ≤ 35 mM and NH4+/Ca+ ≤ 0.8.
Journal ArticleDOI
A Dark Incubation Period Is Important for Agrobacterium-Mediated Transformation of Mature Internode Explants of Sweet Orange, Grapefruit, Citron, and a Citrange Rootstock
Mizuri Marutani-Hert,Kim D. Bowman,Greg McCollum,T. Erik Mirkov,Terence J. Evens,Randall P. Niedz +5 more
TL;DR: These results confirm previous reports on the feasibility of transforming mature tissue from sweet orange and are the first to document the transformation of mature tissues from grapefruit, citron, and a trifoliate hybrid.
Journal ArticleDOI
Mineral nutrition and in vitro growth of Gerbera hybrida (Asteraceae)
TL;DR: Because mineral nutrient effects varied significantly with the response measured, defining an appropriate formulation requires a clear definition of “optimal” growth.
Journal ArticleDOI
Protoplast isolation and plant regeneration of guava (Psidium guajava L.) using experiments in mixture-amount design
TL;DR: Osmolarity was the primary determinant for protoplast yield and viability, irrespective of osmoticum type and K was more effective than BAP in shoot regeneration, but due to synergistic blending the response was highest when both were present.
References
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Journal ArticleDOI
An Analysis of Transformations
George E. P. Box,David Cox +1 more
TL;DR: In this article, Lindley et al. make the less restrictive assumption that such a normal, homoscedastic, linear model is appropriate after some suitable transformation has been applied to the y's.
Book
Response Surface Methodology: Process and Product Optimization Using Designed Experiments
TL;DR: Using a practical approach, this book discusses two-level factorial and fractional factorial designs, several aspects of empirical modeling with regression techniques, focusing on response surface methodology, mixture experiments and robust design techniques.
Book
Regression Diagnostics: Identifying Influential Data and Sources of Collinearity
TL;DR: In this article, the authors present a method for detecting and assessing Collinearity of observations and outliers in the context of extensions to the Wikipedia corpus, based on the concept of Influential Observations.
Book
Classical and modern regression with applications
TL;DR: In this article, the authors focus on concepts with a blend between illustrations using real data sets and mathematical and conceptual development and emphasize applications with examples that illustrate nearly all the techniques discussed, including simultaneous influence, maximum likelihood estimation of parameters, and the plotting of residuals.
Journal ArticleDOI
Simultaneous Optimization of Several Response Variables
TL;DR: In this article, the authors present a set of conditions that will result in a product with a desirable combination of properties, which is a problem facing the product development community in general.
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